骨形态发生蛋白对培养胎儿关节软骨细胞代谢的影响及意义

Bone Morphogenetic Protein's Influence upon the Metabolism of Human Embryo Articular Chondrocytes Cultured in Vitro

为了解骨形态发生蛋白（ＢＭＰ）在关节软骨损伤与修复及异位化骨中的作用，本研究观察了部分纯化的牛骨形态发生蛋白（ｂＢＭＰ）和高度纯化的人骨形态发生蛋白（ｈＢＭＰ）对体外贴壁培养原代、反分化人胎儿关节软骨细胞及鼠３Ｔ３成纤维细胞ＤＮＡ、胶原和蛋白多糖合成的影响。结果发现：部分纯化的ｂＢＭＰ和高度纯化的ｈＢＭＰ均不促进原代人关节软骨细胞合成ＤＮＡ和胶原，并显著抑制其蛋白多糖的合成。部分纯化的ｂＢＭＰ对反分化关节软骨细胞和鼠３Ｔ３成纤维细胞的ＤＮＡ合成、胶原合成和蛋白多糖合成却有明显促进作用。因此，本研究认为，ＢＭＰ诱导下的关节软骨细胞不会发生反分化，而有可能进一步向肥大软骨细胞样细胞或成骨细胞样细胞方向分化，并可能使反分化的软骨细胞重新表达软骨细胞表型。

To understand bone morphogenetic protein(BMP)'s act on the injury and repair of articular cartilage and the formation of ectopic osification, partially purified bovine BMP(bBMP)and highly purified human BMP(hBMP)were used to investigate BMP's influence on the synthesis of DNA,collagen and proteoglycan of cultured primary human embryo articular chondrocytes,dedifferentiated chondrocytes and mouse 3T3 fioroblasts.The results showed that partially purified bBMP and highly purified hBMP couldn't promote the synthesis of DNA and collagen of primary human embryo articular chondrocytes but they inhibit their proteoglycan synthesis remarkably.Partially purified bBMP seriously promoted the synthesis of DNA,collagen and proteoglycan of dedifferentiated chondrocytes and mouse 3T3 fibroblasts.These results indicated that chondrocytes induced by BMP couldn't be dedifferentiated.It was possible for BMP to promote chondrocytes'further differentiation towards hypertrophic chondrocyte-like cells or osteoblast-like cells.It was also possible that BMP could make dedifferentiated chondrocytes express chondrocytes'phenotypes again.