筛选后的巢蛋白阳性细胞流式鉴定及诱导分化

Flow Cytometry Analysis and Differentiation Study of Selected Nestin Positive Cells

目的观察胎儿胰腺来源的巢蛋白阳性细胞(符合医学伦理学)是否具有与间充质干细胞类似的表面标志以及多向分化的潜能。方法从胎儿胰腺中分离的巢蛋白阳性细胞筛选后进行扩增,用流式细胞仪分析表面标志,并探讨这类细胞的成脂和成骨的分化潜能。结果筛选纯化后的巢蛋白阳性细胞的表面标志与骨髓间充质干细胞表面标志相似;此类细胞可在体外诱导为脂肪细胞或骨细胞。结论筛选纯化的胎儿胰腺来源的巢蛋白阳性细胞具有一定间充质干细胞的特性。

Objective To verify the hypothesis that selected nestin positive cells derived from human fetal pancreas （according as medical ethnics）have surface markers similar to bone marrow mesenchymal stem cells （MSCs）, and that these cells have muhilineage potential. Method The cell surface markers were determined by flow cytometry, and then the potential that these cells might be differentiated into adipocytes and osteoplasts were explored. Result These cells have similiar surface markers as MSCs of bone marrow origin. These cells was induced to differentiate into adipocytes and osteoplasts. Conclusion Selected nestin positive cells derived from human fetal pancreas have certain characteristics of MSCs.