淋巴细胞微核检测可用作辐射生物剂量计

Lymphocyte micronucleus assay can be used as a biological dosimeter in radiation accident

用外周血淋巴细胞微核检测法对一起６０Ｃｏ源事故及１例非何杰金氏淋巴瘤６０Ｃｏ源全身照射治疗后的生物剂量进行了估算，取得与物理剂量或染色体剂量一致的结果。照后３１天的微核剂量仍能反映实际剂量，认为微核检测可作为生物剂量计用于估算受照者的生物剂量。在事故情况下，为尽早向临床提供剂量数据，可先观察５２小时培养制片标本，计算出初步参考剂量，然后观察７２小时培养制片的标本，给出正式剂量。

We utilized micronucleus (MN) dose-effect calibration curves obtained by cytokinesis-block (CB) method and conventional method to estimate the biological doses to three irradiated persons in a60 Co source accident in Wuhan and one case of nonHodgkin's lymphoma total bodily irradiated with60Co source.The results showed that(1) the doses estimated by micronucleus assay were consistent with those by physical dosimetry and chromosome aberration analysis;(2) the doses estimated 31 days post irradiation were still consistent with those originally estimated to the three accidental cases,although the former were a little lower than the latter.We consider that all of these MNC or MN calibration curves obtained by either CB MN method or conventional MN method could be used as biological dosimeters to estimate the exposure dose in radiation accident.