PCR－SSP快速HLA－DR52组基因分型与临床应用

Rapid genotyping for HLA-DR52-associated group by PCR-amplification

采用顺序特异引物聚合酶链反应技术（ＰＣＲ－ＳＳＰ）对６５例肾移植供受者ＨＬＡ－ＤＲ５２组基因分型，同时与血清学方法对比研究。显示血清学方法错误率高达３６％；而ＰＣＲ－ＳＳＰ分型全部成功，无一例假阳性和假阴性；重复性１００％，总耗时５小时。分型结果经标准细胞株、限制性内切酶分析和寡核苷酸探针杂交等确证，特异性达１００％。结果表明：ＰＣＲ－ＳＳＰ用于ＤＲ５２组基因分型，快速、特异，结果可靠，适合于临床应用。

A comparative study of genotyping for HLA-DR52 group by polymerase chain reaction with sequence-specific primers(PCR-SSP)and the standard serologic typing was made in 65 donorrecipients of cadaveric kidney. All typings were successful with PCR-SSP without false negative or false positive result while 36% of the serological matching were technically unsuccessful or doubtful. The PCR-SSP result was reproducible in 100% and the whole procedure could be acomplished in 5 hours. The specificity of matching as tested by specific means was also l00%. It was concluded that genotyping for HLADR52-associated DR3,DR8,DR11,DR12,DR13 and DRI4 by PCR-SSP was a rapid and accurate matching technique,suitable for clinical practice.