姜黄素对前列腺癌LNCaP细胞PSA生成及AR表达的影响(英文)

Effects of curcumin on the PSA and expression of AR in prostate cancer cells LNCaP

【目的】研究姜黄素对前列腺癌细胞株LNCaP细胞增殖的影响。【方法】化学发光法检测不同浓度的姜黄素处理前列腺癌细胞株LNCaP后前列腺特异抗原(PSA)含量,利用荧光素酶报告基因pGL-3构建含PSA基因5’侧启动子区640 bp DNA的荧光素酶表达载体pGL3-PSA,并将其转染LNCaP细胞,不同浓度姜黄素作用24 h后应用荧光素酶测定系统检测荧光素酶表达活性。用免疫印迹Western-blotting技术检测雄激素受体(AR)的表达。【结果】姜黄素抑制LNCaP细胞培基中PSA蛋白及含PSA启动子的荧光素酶活性的表达;形态学结果显示姜黄素可抑制前列腺癌细胞LNCaP的增殖;Western-blotting技术检测结果显示姜黄素抑制AR的表达,并且对AR表达的抑制程度依赖于姜黄素的浓度。【结论】姜黄素对PSA启动子的影响及PSA蛋白表达的抑制作用是通过抑制雄激素受体(AR)的表达实现的,姜黄素能够抑制前列腺癌细胞LNCaP的增殖。

[Objective] To observe the effects of curcumin on the proliferation of prostate cancer cell line LNCaP. [Methods] AXSYMTM system - chemical luciferase method was used to examine the content of prostate specific antigen （PSA） in cultures of prostate cancer cell lines LNCaP treated by different dosage of curcumin, pGL3-PSA luciferase expression vector, containing 640 bp DNA of PSA gene 5＇ promoter region was constructed and transfected into the LNCaP cell with lipofectin. Through detecting the activity of lueiferase, the effect ofeurcumin on the promoter of PSA was studied. Western - blotting was used to measure the expression of androgen receptor （AR） in LNCap cells treated with differ- ent concentration of curcumin. [Results] The results showed that expression of PSA was inhibited, activity of luciferase was reduced by curcumin. There were also significant difference in AR expression in LNCaP cells treated by different con- centrations of curcumin. [Conclusion] By inhibiting AR expression, curcumin reduces the function of PSA promoter and inhibits PSA orotein exoression.