高危性人乳头瘤病毒与抑癌基因P53在膀胱癌中的表达

High risk human papillomavirus and P53 protein expression in bladder cancer

用ＰＣＲ法检测５２例膀胱癌组织中人高危性乳头瘤病毒（ＨＰＶ）ＤＮＡ，同时用免疫组化方法检测其Ｐ５３蛋白的过度表达，发现２８例（５３．８４％）ＨＰＶＤＮＡ阳性，１９例（３６．５４％）肿瘤细胞核Ｐ５３染色阳性，二者同时阳性者３例（５．７７％）。ＨＰＶＤＮＡ阳性者多见于高分化、非侵袭性肿瘤中，相反Ｐ５３蛋白阳性则主要表现在低分化、侵袭性肿瘤中。ＨＰＶＤＮＡ和Ｐ５３的表达呈显著性负相关（ｒ＝－０．５７６９）。结果提示：ＨＰＶ感染或Ｐ５３的过度表达与膀胱肿瘤的生物学行为有关，并可将其作为膀胱肿瘤的预后评价指标。

High risk human papillomavinis(HPV)was assayed by polymerase chain reaction and P53 protein expression by immunohistochemistry in 52 cases of transitional cell carcinoma of bladder. HPV DNA was positive in 28(53.84%)and P53 protein expression in 19(36.54 %).HPV DNA and P53 protein were both detected in 3(5.77%).HPV DNA was frequently observed in low grade and noninvasive tumors while P53 more common in high grade and invasive tumors.The result suggested that HPV and P53 protein over expression might serve as a tumor marker for the evaluation of the prognosis of bladder carcinoma.