睾丸肿瘤DNA倍体及HLA－I抗原检测的预后意义

DNA ploidy and HLA class I antigens in testicular tumors

运用流式细胞仪（ＦＣＭ）和免疫组化技术对５５例睾丸肿瘤ＤＮＡ倍体和ＨＬＡ－Ｉ抗原表达进行分析。３例二倍体肿瘤均存活，异倍体肿瘤中非三倍体肿瘤３４例，死亡７例，平均存活时间４１．２个月，三倍体肿瘤１６例，死亡１０例，平均存活时间１５．５个月（Ｐ＜０．０５），ＨＬＡＩ－抗原阳性者２７例，死亡４例，平均存活４０．０个月，阴性者２５例，死亡１３例，平均存活１２．７个月（Ｐ＜０．０５），综合二者进行评估时，ＨＬＡ－Ｉ抗原阴性的三倍体肿瘤预后最差（Ｐ＝０．００１）。提示多参数综合分析在肿瘤预后预测中有十分重要的实用价值。

DNA ploidy and expression of HLA class I antigen were studied by flow cytometry and immunohistochemical technique in 55 paraffin embeded specimens of testicular tumors and in 10 normal testes from BPH as controls.DNA aneuploidy has been an important marker for the diagnosis and proguosis of testicular tumors.Reduction of the HLA class I antigen denoted a more aggressive testicular tumor suggesting that the testicular tumor might escape from immue surveillance,Diploid tumors had the best prognosis and the prognosis of nontriploid tumors was superior to that of triploid tumors in the aneuploid group(P<0.05).The prognosis of negative HLA class I antigen expression tumors was poorer than the postive expression tumors(P<0.05)and tripfoid tumors with negative HLA class I antigen expression had the worst prognosis(P=0.001).Multiparmetric analysis plays an important role in the evaluation of the prognosis of testicular tumors.