人脐血细胞分化为神经细胞的体内实验研究

Progress in human umbilical cord blood cells differentiating into nerve cells in vivo

目的:人脐血富含干细胞和前体细胞,本文检索了人脐血治疗中枢神经系统疾病的体内实验研究状况、脐血细胞穿过血脑屏障的机制,并对其临床应用前景进行展望。资料来源:应用计算机检索PUBMED1997-01/2005-10期间的相关文章,检索词为“Humanumbilicalcordbloodcells,nervecell”,并限定文章语言种类为English。同时计算机检索万方数据库2000-01/2005-10期间的相关文章,检索词为″脐血细胞,神经细胞″,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与人脐血细胞的研究相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到230篇相关文献,52篇文献符合纳入标准,排除的178篇文献为内容陈旧或重复。符合纳入标准的52篇文献中,36篇涉及人脐血治疗中枢神经系统疾病的体内实验研究状况,11篇涉及脐血细胞穿过血脑屏障的机制,5篇涉及人脐血细胞存在的问题与展望。资料综合:脐血富含干细胞和前体细胞,静脉注射人脐血细胞可进入脑内分化为神经干细胞、神经元和星形胶质细胞,提高脑和外周血神经营养因子水平,促进神经功能恢复。脑损伤后,病变组织表达趋化蛋白和黏附分子并释放多种化学物质及一系列生物活性因子,促使脐血中的骨髓间充质细胞、单个核细胞和造血细胞穿过损伤的血脑屏障进入脑内,向病灶迁移通过促进病灶周围的血管再生从而促进神经再生。脐血静脉移植为治疗神经退行性疾病提供了广阔的前景,脐血来源的干细胞可能将成为组织工程中的种子细胞,将对神经系统的细胞治疗和基因治疗起到极大地推动作用。结论:静脉注射人脐血细胞可进入脑内并分化为神经细胞,对神经功能缺损有改善作用,为脐血移植治疗神经系统疾病提供了新思路。

OBJECTIVE： Human umbilical cord blood cells （HUCBC） are rich in stem cells and progenitor cells. We searched the study of HUCBC in nervous disease; the mechanism of HUCBC through blood brain barrier as well as the outlook of HUCBC apply in clinic. DATA SOURCES： We searched for the related articles published between January 1997 and October 2005 in PUBMED on the computer, with the key words of ＂Human umbilical cord blood cells,nerve cell＂ in English .Meanwhile, we also searched for the related articles in Wanfang database on the computer with the key Words of ＂Human umbilical cord blood cells ,nerve cell＂ in Chinese. STUDY SELECTION： The articles were selected primarily and the attached references were also look through. Inclusive criteria： the articles were related to HUCBC. Exclusive criteria： repeated studies or Meta articles were excluded. DATA EXTRACTION： Among 230 selected articles, 52 articles met inclusive criteria. 178 articles were excluded due to dated or repetitive content. Among the 52 articles which met the inclusive criteria, 36 were related to the study status of in vivo experiment on HUCBC for treatment of nervous system.disease, 11 related to the mechanism of HUCBC permeating through blood brain barrier, 5 related to the problems and prospect of HUCBC. DATA SYNTHESIS： HUCBC were rich in stem cells and progenitor cells. HUCBC injected intravenously could enter the brain and differentiate into neural stem cell, neurons and astrocytes. Injection of HUCBC could elevate the level of neurotrophic factor of brain and peripheral blood and improve the neural function recovery. The injured brain tissues expressed chemotactic protiens and adhesion molecule and secreted various chemical materials as well as a series of bioactivity materials that induced the migration of bone marrow-derived mesenchymal stem cells, mononuclear cell and hematopoietic cell into the brain through blood brain barrier to focus. Neovascularization may be contribute to neuronal regeneration. Transplantation of HUCBC provides a good prospect for treatment of nerve degenerative disease. Stem cells from human umbilical cord blood might be the seed cells of tissue engineering, which plays a great promoting role in the cell and gene treatment of nervous system. CONCLUSION： Intravenous injection of HUCBC can enter the brain, differentiate into nerve cell and improve nerve function injury.. HUCBC transplantation will provide a new way in treating disease of nervous system.