慢性压迫性脊髓损伤后神经前体细胞的增殖(英文)

Proliferation of neural progenitor cell after chronic compressive injury of spinal cord

背景:关于成年哺乳类动物脊髓损伤后神经前体细胞的增殖特征和来源以及星形胶质细胞在其中的作用尚无肯定性结论。目的:通过分析成年大鼠慢性压迫性脊髓损伤及减压后巢蛋白和胶质纤维酸性蛋白表达的变化,探讨神经前体细胞的增殖特征和来源以及星形胶质细胞在其中的作用。设计:完全随机对照实验。单位:兰州大学第二医院骨科研究所。材料:实验于2003-03/10在兰州大学第二医院骨科研究所完成,选择成年健康Wistar大鼠50只。随机分为正常对照组、慢性压迫性脊髓损伤中度组(压迫物占椎管矢状径的40%)、重度组(压迫物占椎管矢状径的60%)及重度压迫损伤24h后减压3d、10d组,每组10只。主要观察指标:①各组大鼠压迫临近段(距压迫边缘至5mm)脊髓灰质和白质内nestin的阳性表达并测量其灰度值。②各组大鼠脊髓内胶质纤维酸性蛋白的表达。结果:50只大鼠均进入实验分析。①中度压迫组(白质235.33±6.48,灰质196.28±6.55)、重度压迫组(白质190.45±4.91,灰质173.15±5.98)及重度压迫损伤减压后3d组(白质198.39±3.24,灰质180.38±4.51)和减压后10d组白质(202.55±3.54)中巢蛋白均有明显表达(P<0.05),以重度压迫组最为显著(P<0.01)。减压10d组的灰质和脊髓中央管室管膜细胞的巢蛋白表达与正常对照组相比,差异无显著性意义(P>0.05)。②与正常对照组相比,各损伤组脊髓内胶质纤维酸性蛋白表达增强,胶质纤维酸性蛋白阳性细胞数目增多、胞体肥大,突起增粗、增长。结论:成年大鼠慢性压迫性脊髓损伤及减压后早期存在神经前体细胞的增殖。星形胶质细胞参与神经前体细胞的增殖与迁移,对脊髓具有重要的营养修复作用。

BACKGROUND： There is still no affirmative conclusion on the proliferative characteristics and the sources of neural progenitor cells after chronic compressive injury of spinal cord in adult mammals and the effects of astrocytes in this process. OBJECTIVE： To investigate the proliferative characteristics and the sources of neural progenitor cell and the effects of astrocytes by means of analyzing the changes of expression of nostin and glial fibrillary acidic protein after chronic compressive injury of spinal cord and after decompression in adult rats. DESIGN： Completely randomized control trial. SETTING： Orthopaedics Research Institute, the Second Hospital of Lanzhou University. MATERIALS： The experiment was completed in Orthopaedics Research Institute of the Second Hospital of Lanzhou University from March to October 2003. A total of 50 adult healthy Wistar rats were selected and randomly divided into normal control group, moderate chronic compressive spinal cord injury group （compressive mass occupied 40% of the diameter of spinal canal）, severe compression group （compressive mass occupied 60% of the diameter of spinal canal）. Three-day and 10-day decompression groups （depression after 24-hour severe compressive injury） with 10 in each group. MAIN OUTCOME MEASURES： ① Grey value of positive expression of nestin in grey and white matter in spinal cord segment near compression （5 mm to the edge of compression） in rats of each group. ② Expression of glial fibfillary acidic protein in spinal cord of rats in each group. RESULTS： All the 50 rats entered experimental analysis. ①There were significant expressions of nestin in moderate compression group （white matter 235.33±6.48, grey matter 196.28±6.55）, severe compression group （white matter 190.45±4.91, grey matter 173.15±5.98）, 3-day decompression after severe compressive injury group （white matter 198.39±3.24, grey matter 180.38±4.51） and 10-day decompression group （white matter 202.55±3.54） （P 〈 0.05）, especially in severe compression group （P 〈 0.01）. Compared with the normal control group, the difference between the ex pression of nestin in grey matter and that in ependymal cells on the central canal of spinal cord in 10-day decompression group has no significance （P 〉 0.05）.②Compared with normal control group, the expression of glial fibrillary acidic protein in spinal cord increased in each injury group, and the amount of positive cells of glial fibrillary acidic protein went up and cell soma was hypertrophic, and the processes became thicker and longer. CONCLUSION： There is neural progenitor cell proliferation in the early stage of chronic compressive injury of spinal cord and after decompression in adult rats. Astroeyte participates in proliferation and migration of neural progenitor cells and has important trophic and repair effects on spinal cord.