紫外激光诱导组织自体荧光光谱技术诊断喉癌的研究

The study of UV-laser inducing autofluorescence of tissues spectroscopic technique in the diagnosis of laryngeal cancer

采用308nmXell准分子激光和4400 Boxcar信号处理系统,对正常和癌变的喉组织各27份样品,在310～550nm范围内紫外激光诱导的自体荧光光谱进行分析。结果显示,癌组织与正常组织的荧光光谱的主要区别是460 nm附近次峰强度的不同,从而导致340 nm和440 nm两处相对荧光强度比差异。正常组织和癌变组织I340/I440值分别为0.970±0.186,2.445±0.924(P<0.01)。通过对光谱进行逐步多元线性回归分析,得到了以3个波长为基础的激光诱导荧光(LIF)得分。喉正常组织和癌组织的平均得分分别为+0.935±0.3,-0.935±0.23(P<0.001)。由此建立了组织自体荧光光谱技术区分正常组织和喉癌组织的两个方法。

The autoftuorescence spectroscopy of the tissue induced by UV-laser(310~550nm) was studied using xellexcimer laser(308nm) and 4400 Boxcer signal analyzer in the tissues of 27 normal and 27 laryngeal cancer. Resluts showed that the primary difference of the emission spectra is the intensity difference of the second peak around 460nm,which leads to the difference of ratio 1340/1440. The mean ratio was 0. 97±0. 186 in normal tissues and 2. 445±0. 924 in laryngeal cancers(P<0. 01). A score based on three wave lengths was derived by stepwise multivariatelinear regression analysis of the spectra. The mean score was +0. 935±0. 3 in normal tissues and -0. 935 ±0. 23 in laryngeal cancers(P<0. 001). Thus,we established two ways to distinguish laryngeal cancers from normal laryngeal tissues by autoftuorescence spectroscopic technique of tissues.