鸡内耳毛细胞标志物的比较研究

Comparison study of hair cell markers in chicken inner ear

目的:为研究内耳毛细胞发育和再生提供有价值的标志物。方法:新生鸡内耳冷冻切片,间接免疫荧光细胞化学技术染色毛细胞抗原(HCA)、Espin和肌浆球蛋白7a基因,观察上述基因在毛细胞中的表达部位和强度。结果:HCA、Espin和肌浆球蛋白7a在听毛细胞和前庭毛细胞均表达;HCA染色毛细胞顶部和纤毛,Espin染色毛细胞纤毛和皮板,肌浆球蛋白7a为典型的细胞质染色。结论:HCA、Espin和肌浆球蛋白7a基因是研究毛细胞发育和再生的理想标志物,结合肌动蛋白-F染色毛细胞纤毛可以更好地定性和定量研究内耳毛细胞发育和再生。

Objective：To supply valuable markers for hair cell development and regeneration. Method：Cryosections of new-hatched chicken inner ear were made. The position and intensity of Hair Cell Antigen, Espin, and MyosinTa expression were detected by indirect immunocytochemistry. Result： Hair Cell Antigen, Espin, and MyosinTa were all expressed in both auditory and vestibular hair cell. Hair Cell Antigen was located in the apical part of hair cell and hair cell bundles. Espin was expressed in the hair cell bundles and cuticular piate. MyosinTa immuno-staining resulted in the typical cytoplasm staining. Conclusion.. Hair Cell Antigen, Espin, and MyosinTa are ideal markers for hair cell and can be used for studying the hair cell development and regeneration. These markers combined with F-actin staining could give a better result for qualitative and quantitative investigation for hair cell development and regeneration.