黄芪对心肌炎小鼠心肌穿孔素mRNA和CVB3m mRNA表达的影响

Influence of Astragalus on expression of perforin and CVB3m in myocardium with acute myocarditis caused by coxsackievirus B3

目的探讨黄芪注射液治疗小鼠柯萨奇B3(CVB3)病毒性心肌炎(VMC)的疗效及其机制。方法BALB/c小鼠24只腹腔感染柯萨奇病毒B3亲心肌细胞株(CVB3m)后,随机等分为两组:黄芪治疗组(黄芪注射液每天10g/kg腹腔注射)和对照组。实验第8天留取心肌,分别进行心肌病理检查,心肌穿孔素(perforin,PFP)mRNA和CVB3m mRNA逆转录多聚酶链反应(RT-PCR)分析,并对心肌组织PFP mRNA和CVB3m mRNA表达水平进行相关性分析。结果(1)心肌组织病理改变:黄芪治疗组明显轻于对照组;(2)心肌PFP mRNA表达水平RT-PCR半定量:黄芪治疗组心肌PFP mRNA的表达明显低于对照组(1.10±0.07与1.31±0.12,P<0.01);(3)心肌CVB3m mRNA表达水平RT-PCR半定量:黄芪治疗组心肌CVB3m mRNA表达明显低于对照组(1.07±0.04与1.18±0.02,P<0.01);(4)心肌PFP mRNA水平与CVB3mmRNA水平呈显著正相关(r=0.68,P<0.01)。结论PFP介导的细胞毒性作用在病毒性心肌炎的发病中起重要作用。黄芪对小鼠急性心肌炎模型中心肌组织CVB3m病毒复制有明显的抑制作用,心肌组织中PFP mRNA表达水平与心肌组织CVB3m mRNA的表达水平的变化具有同步性,黄芪对CVB3m诱导的小鼠急性心肌炎模型有显著的治疗作用。

Objective To investigate the effect and mechanism of Astraga/us injection on acute murine myocarditis caused by coxsackieviras B3. Methods Acute viral myocarditis was induced in 24 BALB/c mice by injection of coxsackievirus B3m（CVB3m） intraperitoneally. Half of the mice were administered Astragalus injection （ 10 g/kg every day）. Heart and blood samples were obtained from all mice on the 8th day of CVB3m infection for hispathological study, detection of myocardial perforin and CVB3m expression by reverse transcription-polymerase chain reaction（RT-PCR）. Results （1） Astraga/us-treated mice showed a significant reduction in myocardial lesion as compared with that in untreated mice. （2） Myocardial perforin expression by semi-quantitative RT-PCR in Astraga/us-treated mice was much lower than that in controls（1.10 ± 0.07 vs 1.31 ± 0.12, P 〈 0.01）. （3） Myocardial CVB3m expression shown by semi-quantitative RT-PCR in Astraga/us-treated mice was much lower than that in controls（ 1.07 ± 0.04 and 1.18 ± 0.02, P 〈 0.01. （4） Myocardial perforin expression level showed significant positive correlation with the changes of myocardial CVB3m expression（ r = 0.68, P 〈 0.01 ）. Conclusion Perforin-mediated cytotoxicity may play an important role in the development of viral myocarditis. Astraga/us has therapeutic effects on viral myocarditis. The mechanism may be explained by anti-viral efficacy, improving of circulation and lightening of myocardial injure.