粒-巨噬细胞集落刺激因子对小鼠体内树突状细胞增殖和活化的影响

Effects of GM-CSF on the proliferation and activation of dendritic cells in vivo

目的:研究粒-巨噬细胞集落刺激因子(GM-CSF)在体内对树突状细胞(DC)增殖和活化的影响。方法:将Balb/c小鼠随机分为GM-CSF处理组和对照组,致死量γ射线照射后移植入绿色荧光蛋白(GFP)标记的骨髓来源的造血干细胞(HSC),GM-CSF处理组隔天皮下注射0.1μg直至处死,对照组隔天皮下注射PBS。应用流式细胞仪分析和比较两组移植后1,2,3及4周小鼠脾脏内DC的数目、活化状态和随时间变化的DC动力学。结果:移植后各时间点GM-CSF处理组小鼠脾脏内DC数目明显高于对照组(均P<0.05),其中最大高出倍数为5倍;GM-CSF处理组DC的CD40,CD80和CD86均高于处理组;骨髓移植后第1周,小鼠脾脏内即出现HSC来源的DC,第2周数目开始上升,至第3周达到高峰,第4周开始下降。结论:GM-CSF对体内HSC分化为DC具有促进作用,并且可促进体内DC的成熟与活化。

Objective To explore the effects of granulocyte macrophage-colony-stimulating factor （GM-CSF） on the proliferation and activation of dendritic cells （DC） in vivo. Methods Balb/c mice were randomly assigned into the control and GM-CSF treatment group. After the lethal dosage of irridiation, green fluorescence protein labelled hematopoietic stem cells （HSC） were injected into the tail veins of the mice. The dosage of 0.1μg GM-CSF was administer subcutaneously every 2 days after the HSC infusion. The numbers and activation status of splenic DC were observed by flow cytometry 1,2, 3, and 4 weeks after the HSC transplantation. Results ment group were significantly higher than those of the The numbers of splenic DC in the GM-CSF treatcontrol group. The expressions of surface marker CD40, CD80, and CD86 in the GM-CSF treatment group were also higher than those of the control group. Conclusion GM-CSF can enhance the proliferation and activation of DC in vivo.