小麦条锈菌国际鉴别寄主Vilmorin23的抗条锈病基因YrV23微卫星标记

A Microsatellite Marker Linked to the Stripe Rust Resistance Gene YrV23 in the wheat variety Vilmorin23

Vilmorin23是小麦条锈菌国际鉴别寄主和国际上重要抗源材料。采用SSR技术,利用由Vilmorin23为基因供体转育而成的小麦抗条锈近等基因系Taichung29*6/YrV23,选用YrV23所在2B染色体上的55对SSR引物,对Taichung29*6/YrV23及其轮回亲本Taichung29和抗性基因供体Vilmorin23的基因组DNA进行PCR扩增和聚丙烯酰胺凝胶电泳分析。结果显示,引物Xwmc356在近等基因系与轮回亲本间扩增出特异性DNA片段,经F2代群体150个抗、感单株检测证实,该片段位点与抗条锈病基因YrV23有连锁关系,遗传距离为9.4 cM。Xwmc356可作为抗条锈基因YrV23的SSR标记。

Vilmorin23 is an internationally used differential host variety for studies on the interactions between wheat stripe rust and wheat, It contains the stripe rust resistance gene YrV23 and is potentially an important source of stripe rust resistance worldwide. SSR analysis was performed on the wheat NIL Taichuang 29 ＊ 6/YrV23 carrying the resistant gene YrV23 against stripe rust, Vilmorin 23 and its recurrent parent Taichung 29. Fifty-five pairs of SSR primers on wheat chromosome 2B were screened and a reproducible polymorphic DNA fragment amplified by Xwmc356 was found. Genetic linkage was tested on 150 segregating F2 plants. It showed that the microsatellite marker Xwmc356 was linked to the resistance gene YrV23 with a genetic distance of 9.4 cM.