摘要
目的:建立单克隆抗体(McAb)夹心ELISA法,用于检测SARS病毒(SARS-CoV)抗原。方法:用间接夹心ELISA法筛选捕捉和标记用单克隆抗体的组合,采用过碘酸钠法标记辣根过氧化物酶(HRP),优化后用于检测SARS-CoV。结果:从12株抗SARS-CoV鼠单克隆抗体中筛选出2A3/1C5组合用于捕捉SARS-CoV,1A5/1B4组合标记HRP作为指示抗体。优化后2A3/1C5的最适工作浓度为1∶4000,HRP-1A5/1B4的最适工作浓度为1∶2000。本方法检测SARS-CoV的敏感度为105pfu/mL。结论:单克隆抗体夹心ELISA法可特异性检测SARS-CoV抗原。
Objective： To establish an assay for SARS-CoV by McAbs sandwich ELISA method. Methods： The McAbs for this assay were selected by indirect sandwich ELISA and the McAbs were labeled by HRP. After optimization, McAbs sandwich ELISA method was used for detection of SARS-CoV. Results： Combined 2A3/1C5 and 1A5/1B4 were selected from a panel of twelve McAbs established by immunizing BALB/c mice with inactivated BJ01 strain. The working concentration of the capture McAbs 2A3/1C5 was 1：4 000 and the HRP-1A2/1B4 was 1：2 000. The sensitivity of this assay was 105 pfu/mL. Conclusion： McAbs sandwich ELISA assay could be used specifically for detection of SARS-CoV.
出处
《生物技术通讯》
CAS
2006年第1期34-36,共3页
Letters in Biotechnology
