16S rRNAPCR鉴定脆弱类杆菌

The Identification of Bacteroides fragilis by 16S rRNA PCR

目的:应用16SrRNA序列设计PCR引物鉴别脆弱类杆菌。方法:通过脆弱类杆菌16SrRNA序列特异性位点设计引物,对4株脆弱类杆菌及大肠杆菌、乳酸杆菌、嗜热链球菌等进行PCR扩增。应用琼脂糖电泳法对PCR扩增产物进行特异性检测。结果:脆弱类杆菌在176bp左右出现特异性条带,而其他细菌均未出现特异性条带。结论:通过16SrRNA序列中特异位点设计引物进行PCR,可特异性鉴定脆弱类杆菌。

Objective： To identify Bacteroides fragilis by 16S rRNA PCR. Method： 4 strains of B.fragilis and 3 strain of other bacteria were identified by 16S rRNA PCR, the special primer of B.fragilis was designed that based on 16S rRNA of B.fragilis. PCR products were confirmed by agrose gel electrophoresis. Results： The 176 bp special straps of B. fragilis appears as expected, while the other kinds of bacteria do not appear the strap. Conclusion： 16S rRNA-targeted primer can be applicable for the reseach of identification and quantification of B.fragilis.