近红外漫反射光谱法快速无损鉴别阿胶真伪

Fast and Nondestructive Discrimination of Donkeyhide Glue by Near-Infrared Spectroscopy

采用近红外光谱漫反射光谱技术和模式识别技术快速鉴别阿胶真伪。收集来源不同的阿胶(真品8个,伪品6个),采集其近红外漫反射光谱,使用多重散射校正和小波变换对光谱进行预处理后,分别应用相似度匹配和马氏距离方法建立质量鉴别模型。相似度法使用真品谱图作为标准谱图,用样品谱图与标准谱图的相似度值来鉴别阿胶质量;对阿胶样品进行重复扫描得到28张谱图,随机分为3组后应用马氏距离法建立交叉验证鉴别模型。两种模式识别方法均能准确无误的鉴别阿胶真伪,表明近红外光谱和模式识别技术结合可快速、准确、客观地进行阿胶质量鉴别,可推广到其他中成药的质量鉴别。

Near-infrared （NIR） diffuse reflectance spectroscopy and pattern recognition techniques were applied to develop a fast identification method of a Chinese patent medieine-donkeyhide glue. Samples from different manufactures （eight genuine samples and six counterfeits） were collected, and their NIR spectra werc obtained. NIR spectra were pretreated with multiplieative signal correction （MSC） and wavelet transformation to diminish ba.seline offset. Similarity and Mahalanobis distance methods were separatemy used to qualify donkeyhide glue. For the similarity calculation, spectra of the two real ones were selected as standards, and then others were compared with the standards to obtain the match value. All the samples were rescanned once for the Mahalanobis distance methods, and totally twenty eight spectra were separated into three sets for cross-valldation, The two methods can both distinguish the counterfeits of donkeyhide glue successfully. The proposed method is accurate and robust, and could be used in the discrimination of other traditional Chinese medicines.