四溴荧光素与DNA作用的研究

Study on the Interaction of Tetrabromofluorescein and DNA

用荧光光谱和磷光光谱法研究了四溴荧光素(TBF)与DNA的相互作用。探讨了盐效应和乙醇在DNA存在和不存在条件下对TBF体系荧光强度的影响,以及不同pH在DNA存在和不存在条件下对体系磷光强度的影响。由荧光猝灭实验,得到无DNA存在下的荧光猝灭常数为719.74L·mol-1,有DNA存在下的荧光猝灭常数为880.22L·mol-1。并通过荧光猝灭实验和磷光偏振实验得出了TBF和DNA的可能作用方式。

The interaction of tetrabromofluorescein （TBF） and DNA was studied by the fluorescence and SS-RTP. The effect of the ion strength and ethanol on the fluorescence spectra of TBF in the presence of DNA and absence of DNA was investigated. In addition, the effect of pH on the SS-RTP spectra of TBF was also examined. The quenched constant was 719.74 and 880.22 in the absence and presence of DNA respectively. Finally, the interaction mode between TBF and DNA was discussed from the fluorescence quenched and phosphorescence polarized experiments.