摘要
本文通过紫外线诱变获得产脂肪酶菌株ＵＶ_７－２，使其脂肪酶活力比原始菌株提高了３０％。同时对该酶的酯化性质进行了研究，并用正交试验方法确定了合成油酸正丁醇酯的最佳条件。最佳条件为温度３５℃，时间１０ｈ，加酶量为３００μ（约干细胞粉０．８５ｇ）。
In this study,a mutant UV7-2with lipase activity is obtained by ultraviolet.Its lipase activity in creases.from 135μ/g to 175μ/g.Esterifacation by the Lipase is also studied.The optimal conditios for the synthesis of oleic acid and n-butanol are obtained by single factor examination as well as orthogonal experiment.The optimal temperature is 35℃;time 10hr;enzyme volume 300μ.
出处
《大连轻工业学院学报》
1996年第1期65-68,共4页
Journal of Dalian Institute of Light Industry
