摘要
本文的目的是研究沙棘叶中的多糖组分.采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖的方法,用酸性乙醇和DEAE_SephdexA_25进行分离纯化,红外光谱鉴定多糖,纸层析及气相色谱分析其单糖组成.结果证明:纯度鉴定表明SJ21为纯多糖,分子量约为70KD.SJ21红外光谱中有典型的多糖和β型糖苷键吸收峰.纸层析及气相色谱分析其单糖组成为Xyl、Ara、Man、Glc、Gal,摩尔比为1.0∶2.1∶2.9∶5.3∶3.7.SJ21为首次从沙棘叶中提取获得.
The combination of NAA and 6-BA was better for the callus induction of Saussurea involucrata than single NAA, 6 - BA, and 2,4 - D. MS medium was most effective for callus induction from leaves; 6,7 - V medium was most effective for adventitious buds induction from callus; 90 % of the adventitious buds rooted well after they were planted on the 1/2 MS containing O.Smg＇L^-1 NAA for 28 days.
出处
《中央民族大学学报（自然科学版）》
2006年第1期22-25,29,共5页
Journal of Minzu University of China(Natural Sciences Edition)
基金
中央民族大学211项目资助
关键词
沙棘叶
多糖
分离纯化
组成
callus induction
adventitious buds
adventitious roots
plantlet regeneration
Saussurea involucrata
