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基于Nafion-二茂铁膜修饰膜的H_2O_2生物传感器 被引量:2

Biosensor based on nation-ferrocence film for H_2O_2 assay
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摘要 该文通过 Nafion、戊二醛和牛血清蛋白的交联,将电子传导体二茂铁和辣根过氧化物酶固定在玻碳电极上,制成了一种过氧化氢生物传感器。这种新型过氧化氢生物传感器性能稳定、灵敏度高,过氧化氢在1.0×10^(-4)~8.0×10^(-3) mol/L 的浓度范围内与传感器的电流强度呈线性关系。 In this paper, ferrocene as the electron transfer and horseradish peroxidase were immobilized on the glassy carbon electrode by nation film, glutaraldehyde, and bovine serum albumin to develop a novel H2O2 biosensor with a stable and sensitive characteristic. The concentration of H2O2 has a linear relationship with the current of the biosensor ranging from 1.0×10^-4 to 8.0×10^-3 mol/L.
出处 《化学传感器》 CAS 2005年第4期22-25,共4页 Chemical Sensors
关键词 生物传感器 nafion-二茂铁膜 过氧化氢 biosensor nation - ferrocence film H2O2
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  • 1Matsubara C, Kawamoto N, Takamura K. Oxo [5, 10, 15,20 - tetra(4 - pyridyl)poyphyrinato] titanium (IV) - An ultra - high sensitivity spectrophotometric reagent for hydrogen peroxide[J]. Analyst, 1992, 117:1781 - 1 784.
  • 2Nakashima K, Maki K, Kawaguchi S, et al. Peroxyoxalate chemiluminescence assay of hydrogen peroxide and glucose using 2, 4, 6, 8-Tetrathio- morpholinopyrimido[5, 4 - d] pyrimidine as a fluorescent component[J]. Anal. Sci., 1991, 7:709-714.
  • 3Oungpipat W, Alexander P W, Southwell - Keely P. A reagentless amperometric Biosensor for hydrogen peroxide determination based on asparagus tissue and ferrocene mediation[J]. Anal. Chim. Acta, 1995, 309:35 -45.
  • 4Fortier G, Vaillancourt M, Belanger D. Evalution of nafion as media for glucose oxidase immobilization for the development of an amperometric glucose biosensor[J].Electroanalysis, 1992, 4:275 - 283.
  • 5Sun C Q, Li W J, Sun Y P, et al. Fabrication of multilayer films containing horseradish peroxide sensor[J]. Electrochimica Acta, 1999, dd: 3 d01 - 3 407.
  • 6Goudaa M D, Kumarb M A, Thakura M S, et al. Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents[J]. Biosensors and Bioelectronic, 2002, 17:503 - 507.
  • 7Garjonyte R, Malinauskas A. Electrocatalytic reactions of hydrogen peroxide at carbon paste electrodes modified by some metal hexacyanoferrates[J]. Sensors and Actuators B. 1998, 46:236-241.

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