金属离子对法夫酵母产虾青素影响的研究

Studies on Effects of Metal Ions on Astaxanthin Production by Phaffia rhodozyma

为了研究金属离子对法夫酵母产虾青素的影响并优化出有利于法夫酵母产虾青素的金属离子,用单因子实验方法研究不同离子对法夫酵母产虾青素的影响并用正交实验方法对金属离子进行了配比优化。在培养基中添加Zn2+和Mn2+能增加细胞干重,在培养基中添加Zn2+、Fe3+、Mn2+和Cu2+能使培养液中虾青素的浓度增加,在培养基中添加Fe3+和Cu2+能使细胞中虾青素的含量增加。在培养基中添加3μmol/L的Mn2+、1μmol/L的Zn2+、1μmol/L的Fe3+、5μmol/L的Cu2+有利于法夫酵母虾青素的合成,用这些金属离子批式培养法夫酵母,总细胞得率为0.349 g/g(葡萄糖),总虾青素得率为0.266 mg/g(葡萄糖),细胞内虾青素最高含量为0.81 mg/g。

Single factor comparison experiment and orthogonal experiment were adopted to study the el＇feels of several metal ions on astaxanthin accumulation by Phffia rhodozyma and optimize metal ion composition for the yeast producing astaxathin. Different metal ion would lead to different results for cultivations of Phaffia rhodozyma, Zn^2＋ and Mn^2＋ at low concentration added into the media would lead to increasing biomass, Zn^2＋ , Fe^3＋ , Mn^2＋ and Cu^2＋ at low concentration added into the media would lead to increasing astaxanthin concentration in culture medium. Fe^3＋ and Cu^2＋ at low concentration would lead to increasing astaxanthin concentration in cells. The optimal metal ion content for promoting astaxanthin accumulation gained by orthogonal experiment was composed with 3μmol/L Mn^2＋ , 1μmol/l Zn^2＋ , 1μmol/l Fe^3＋ and 5μmol/l Cu^2＋ . In a batch culture of Phaffia rhodozyma in the presence of the adore optimized metal ion composition, the yield of total cells （dx/ds, g/g）, product （dp/ds, mg/g） and the content of pigment in cell（dp/dx, mg/g） were 0.349g/g , 0.266mg/g and 0.81mg/g, respectively.