藜蒿中绿原酸的密闭微波辅助萃取研究

Study on Airtight Microwave-assisted Extraction of Chlorogenic Acid in Artemisia selengensis Turcz

应用具有温度控制附件的密闭微波萃取装置,通过正交试验,对藜蒿茎中绿原酸进行微波萃取研究。讨论了提取溶剂浓度和用量、微波提取时间和微波功率对微波萃取的影响。实验表明,以体积分数50%的乙醇水溶液为提取剂,原料:提取剂(g∶mL)=1∶20,在微波功率400 W和温度70℃下萃取12 min,绿原酸的提取率最佳。此外,将微波萃取与乙醇回流提取进行了比较。

Using an airtight microwave - assisted extraction apparatus with a temperature controlling part, a microwave - assisted extraction procedure was developed for chlorogenic acid in stem of arteisia selengensis turcz by an orthogonal test method. The effects of solvent concentration and volume, microwave radiation time and power on extraction output were investigated. The optimum conditions of extraction were obtained as follows：50 % alcohol as solvent, the 20 times volume of sample weight, microwave power 400 W and extraction for 12 min at 7012. Compared with alcohol refluxing extraction,microwave assisted extraction is more efficient.