一种蛛网膜下腔出血后脑血管痉挛的动物模型

A kind of Animal Model of Cerebral Vasospasm following Subarachnoid Hemorrhage

目的建立一种简便、稳定的脑血管痉挛模型。方法取新西兰大白兔18只,随机分成SAH组、手术对照组、生理盐水组3组,每组6只,每组分别于手术后第3、7、14d行DSA(脑血管造影)、TCD(经颅多普勒超声)、组织学检查。结果TCD检查表明仅SAH组脑血流速度明显增加;组织学检查示SAH组动物脑血管壁均存在不同程度的炎性反应;DSA证实SAH组动物发生了明显痉挛。结论此方法适合制作蛛血后脑血管痉挛模型。

Objective To establish a kind of convenient,stable model of cerebral vasospasm following subarachnoid hemorrhage. Methods Eighteen Jersey rabbits were divided randomly into three goups, SAH group,operation control group and normal sodium control group. DSA ,TCD and histological study were performed in all groups at the third,senventh and fourteenth day after the operation respectively. Results in SAH group,the velocity of cerebral blood flow detected by TCD has greatly risen and there was obvious inflammatory reaction reflected by histological study in vessel wall,the existence of cerebral vasospasm was confirmed by DSA also only in SAH group. Conclusion This method is fit for the establishment of cerebral vasospasm model following SAH.