牛磺酸对大鼠局灶性脑缺血再灌注损伤的保护作用

Protective effect of taurine on reperfusion injury after focal cerebral ischemia in rats

目的研究牛磺酸(Taurine)对大鼠局灶性脑缺血再灌注损伤的影响并探讨其机制。方法采用大鼠大脑中动脉梗死(MCAO)模型,将大鼠随机分为假手术组(Sham)、缺血再灌注组(I/R)和缺血再灌注加牛磺酸处理组(I/R+Taurine),观察缺血1 h再灌注24 h后,各组大鼠脑梗死体积、脑水含量及核因子кB(NF-кB)P65表达的变化。结果3组大鼠脑梗死体积百分比分别为0(、13.32±3.18)%(、9.21±2.24)%,与单纯缺血再灌注组比较,牛磺酸处理组脑梗死体积减少30.83%(P<0.05);脑水含量分别为(79.28±0.66)%,(82.46±0.94)%,(80.73±1.40)%,给药组脑水肿明显减轻(P<0.05);免疫组化染色结果显示牛磺酸抑制NF-кB的表达(P<0.05)。结论牛磺酸对大鼠局灶性脑缺血再灌注损伤具有保护作用,该作用与其抑制NF-кB的激活,减轻神经细胞损害有关。

Objiective To investigate the effect of taurine on reperfusion injury after focal cerebral ischemia in rats and its related mechanism, Methods A middle cerebral artery occlusion model was developed in Wistar rats. They were divided into three groups： sham group, ischemia - repeffusion group（I/R）, and ischemia - reperfusion with taurine treatment group, After ischemia for 1 hour and repeffusion for 24 hours in each model rat, the change in cerebral infarct volume, water content, and the expression of NF - κB P65 were determined. Results The percentages of cerebral infarct volume 0, （ 13.32 ± 3.18） %, and （9.21 ± 2.24） % in the groups 1 - 3, respectively, and it was reduced by 30. 83 % in taurine treatment group, compared with I/R group （ P 〈 0.05 ）. The brain water cantent was（ 79.28 ± 0,66 ） %, （ 82.46 ± 0.94 ）%, and （ 80.73 ± 1. 50 ）% in the three groups with a significant decrease in taurine treatment group （ P 〈 0.05 ）. And likely, the expression of NF- κB P65 protein was down regulated with taurine treatment （ P 〈 0.05 ）. Conclusions Taurine has a neuroproteetive effect on reperfusion injury after focal cerebral ischemia in rats. This effect is related to the inhibition of activation of NF - κB and the alleviation of neuronal damage.