载脂蛋白E基因多态性与原发性高脂血症中医辨证的相关性研究

Study on the Relationship between Polymorphism of ApoE Gene and TCM Syndrome Type of Primary Hyperlipemia

目的探讨载脂蛋白E(ApoE)基因多态性与原发性高脂血症中医辨证的相关关系。方法采用基因测序法检测102例高脂血症患者的ApoE基因型。结果共检出5种基因型,分别是E2/2、E3/3、E4/4、E2/3、E3/4;肝肾阴虚证E3/4+E4/4基因型频率和ε4等位基因频率明显高于脾肾阳虚证和痰浊阻遏证(P<0·05,P<0·01),气滞血瘀证E3/4+E4/4基因型频率和ε4等位基因频率也明显高于痰浊阻遏证(P<0·05)。结论ApoE基因多态性与原发性高脂血症中医辨证具有一定的相关性。

Objective To study the relationship between the polymorphism of ApoE gene and TCM syndrome type of primary hyperlipemia. Methods ApoE genotype of 102 patients with hyperlipemia was detected by gene PCR sequencing. Results A total of five genotypes were detectable, they were E2/2, E3/3, E4/4, E2/3 and E3/4. The frequency of E3/4 ＋ E4/4 and ε4 allelotype detected in the patients of Gan-Shen Yin deficiency syndrome type were significantly higher than those in patients of Pi-Shen Yang deficiency type or of phlegm stagnation type （P 〈 0.05, P 〈 0.01）, and which in patients of Qi-stagnation caused blood stasis type were significantly higher than those in patients of phlegm stagnation type （ P 〈 0 . 0 5 ） . Conclusion Polymorphism of ApoE gene is related in a certain degree to TCM syndrome type of primary hyperlipemia.