鲜壁虎冻干粉抑制H22肿瘤血管生成机理的实验研究

Experimental Study on Mechanisms of Lyophilized Powder of Fresh Gekko Chinenis in Inhibiting H22 Hepatocarcinoma Angiogenesis

目的探讨鲜壁虎冻干粉对H22肝细胞癌血管生成的机制。方法建立移植瘤小鼠H22肝细胞癌模型。将30只小鼠随机分为顺铂治疗组、壁虎治疗组以及对照组,分别给予顺铂腹腔注射(1mg/g),口服鲜壁虎冻干粉(1·2g/kg)及口服等量生理盐水。给药20次后,用肿瘤组织称重法评价抗肿瘤活性,用TUNEL方法检测细胞凋亡率。采用Weidner方法检测肿瘤组织的微血管密度,S-P免疫组化法检测血管内皮生长因子(VEGF)、碱性成纤维细胞因子(bFGF)的蛋白表达。结果鲜壁虎冻干粉可抑制H22小鼠肿瘤生长及诱导肿瘤细胞凋亡,并可降低肿瘤组织VEGF、bFGF蛋白的表达,使肿瘤组织内微血管密度下降。结论鲜壁虎冻干粉可抑制H22小鼠肿瘤生长及血管生成,其机制可能与下调VEGF、bFGF蛋白的表达有关。

Objective To investigate the inhibitory effects and its mechanism of lyophilized powder of fresh Gekko Chinenis （GCLP） on H22 hepatocarcinoma growth and angiogenesis in vitro. Methods The transplant tumor model of H22 hepatocarcinoma in mice was established, Thirty mice were randomly divided into three groups, the cisplatin group, the GCLP group and the control group, they were treated respectively with intraperitoneal injection of cisplatin 1 mg/g, oral administration of GCLP in a dose of 1.2 g/kg, and equal volume of saline, the medication was given for 20 times totally. The anti-tumor activity was evaluated by tumor tissue weighing, the cell apoptotic rate was detected by TUNEL method, the micro-vessel density in tumor tissue was determined by Weidner method, the protein expression of vascular endothelin growth factor （VEGF） and basic fibroblast growth factor （bFGF） were detected by S-P immunohistochemistry. Results GCLP could obviously inhibit the hepatocarcinoma growth, induce tumor cell apoptosis, and reduce micro-vessel density in tumor tissue through down-regulating VEGF and bFGF protein expression. Conclusions GCLP can effectively inhibit the growth of H22 hepatocarcinoma and angiogenesis. Its mechanism might be related to the down-regulation of the protein expression of VEGF and bFGF.