葛根素治疗子痫前期大鼠的疗效观察

Therapeutic effect of puerarin on rats with pre-eclampsia

目的探讨葛根素对子痫前期大鼠的治疗效果。方法选取SD雌性孕鼠共30只,随机分为正常组、子痫前期组和葛根素组(每组10只)。于孕14d,分别给子痫前期组和葛根素组大鼠尾静脉注入内毒素(1.0μg/kg),建立子痫前期模型,葛根素组大鼠同时给予腹腔注射葛根素注射液(30mg·kg-1·d-1)治疗;正常组大鼠尾静脉注入等量生理盐水。孕第8、15、19天测定各组大鼠24h尿蛋白含量;孕第10、15、19天测定各组大鼠心率、血压;孕第21天用硝酸还原酶法测定各组大鼠血浆一氧化氮(NO)浓度。结果(1)葛根素组、子痫前期组、正常组大鼠24h尿蛋白含量(孕第19天尿蛋白含量-孕第8天尿蛋白含量)分别为(73±20)、(464±57)、(140±12)μg,3组两两比较,差异有统计学意义(P<0.01)。(2)3组大鼠血压(孕第19天血压-孕第10天血压)比较,差异也有统计学意义(P<0.01)。(3)3组大鼠心率(孕第19天心率-孕第10天心率)比较,差异无统计学意义(P>0.05)。(4)孕第21天,血浆NO浓度正常组为(158±31)mmol/L,子痫前期组为(49±36)mmol/L,葛根素组为(162±14)mmol/L,子痫前期组分别与其他两组比较,差异均有统计学意义(P<0.01)。结论葛根素可以提高子痫前期模型大鼠体内NO水平,降低血压和尿蛋白含量,对心率没有明显影响。

Objective To study the effect of puerarin on the nitrogen monoxide（NO） concentration in rats with pre-eclampsia. Methods Thirty SD rats were selected and divided into three groups： normal pregnancy group, pre-eclampsia model group and puerarin group（ 10 rats in each group）. We used endotoxin to set up the pre-eclampsia model rats on gestation 14th day and puerarin to treat these rats. The 24 hour urine protein on gestation 8th, 15th, 19th days were determined, as well as the heart rate, blood pressure on gestation 10th, 15th, 19th days. Nitrate reductase method was used to determine the plasma NO concentration of each group on gestation 21st day. Results （1） There was significant difference in urine protein concentration between the puerarin [ （73 ± 20） μg ], pre-eclampsia [ （464 ± 57 ） μg ], and normal pregnancy [ （140 ± 12）μg] , groups （which is equal to the value of urine protein of pregnancy 19th day subtracting that of pregnancy 8th day, P 〈 0. 01 ）. （2） There was significant difference in blood pressure （which is equal to the value of blood pressure of pregnancy 19th day subtracting that of pregnancy 10th day） between the three groups （ P 〈 0. 01 ）. （ 3 ） There was no significant difference in changes of heart rate between the three groups（which is equal to the value of heart rate of pregnancy 19th day subtracting that of pregnancy 10th day, P 〉 0.05 ）. （4）There was significant difference in NO concentration （NO concentration at gestation 19th day subtracting that of 8th day） between the three groups （ P 〈 0.01 ）, and the value of normal pregnancy group was （ 158 ± 31 ） mmol/L, pre-eclampsia model group （49 ± 36 ） mmol/L, puerarin group （ 162 ± 14） mmol/L. Conclusion Puerarin can elevate the level of NO concentration and decrease the blood pressure and urine protein, but not the heart rate in pre-eclampsia model rats, which suggests that puerarin may be used to treat pre-eclampsia.