白藜芦醇对D-半乳糖致衰老小鼠行为记忆功能的影响

Effects of resveratrol on behavior and memory senility caused by D-galactose in rats

目的:观察一定剂量的白藜芦醇对D-半乳糖致衰老小鼠模型的行为记忆功能是否有改善作用。方法:100只小鼠随机分5组,1组为空白对照组,另4组连续注射半乳糖72天,其中1组为衰老组,另3组从第23天天始至第72天分别腹腔注射3个不同剂量的白藜芦醇进行干预。采用Y迷路、Morris水迷宫检测小鼠行为记忆功能,荧光分光分度计测定小鼠脑组织脂褐素的含量。结果:低剂量白藜芦醇组和衰老组相比,检测指标无统计学差异;中高剂量白藜芦醇组织和衰老组织相比,在Y水迷路方向辨别试验中正确达岸次数增加,在Morris水迷路中潜伏期缩短,小鼠的游泳路径缩短,穿越次数增加,但是两组的组间数据无统计学差异。结论:中高剂量的白藜芦醇可以改善D-半乳糖致衰老小鼠的行为记忆功能,具有延缓衰老的作用。

Objective：This study was designed to investigate whether there was influence on behavior and memory senility caused by D-galactose in rats which were injected with resveratrol in a certain dose. Methods：One hundred mice were randomly divided into 5 groups according to injection condition （normal saline,D-galactose,low,middle and high dose of resveratrol）. The D-galactose group and resveratrol group were continually injected with D-galactose for 72 days. And the resveratrol group was continually injected with resveratrol from the 23th day to the 72th day. After these animal models were finished,they were undergone these tests including Y type maze step through test. Morris maze test and lipofuscin gnantitative analysis. Results：There was no statistical difference between low dose of resveratrel and models. Such differences of swimming length and the mumbers through the flat roof in Morris maze and the right numbers in Y type maze through test and the brain lipefuscin content were statistically valid by spss analysis between the D-galactose group and the middle and high dose of resveratrol group （P〈0.05）,but there was no statistical difference between middle and high dose of resveratrol groups, Condusion：The middle and high dose of resveratrel can improve the behavior and memory senility caused by D-galactese,and can inhibit the development of the brain lipofuscin.