摘要
以BLMA5Fe2+体系切割双链DNA产生MDA物质为基础,采用TBA法检测了肌肽对DNA氧化损伤的保护作用,同时以甘露醇、苯甲酸钠为对照,并对肌肽的抗氧化机理做了初步探讨.结果发现肌肽对DNA损伤的保护作用明显优于甘露醇和苯甲酸钠.其抗氧化曲线与L-组氨酸相似,因此推测其抗氧化性与其所含的咪唑环密切相关.
DNA can be cleaved by BLMA5-Fe^2 + system to produce MDA, this DNA damage can be observed by TBA reaction. The antioxidant "abilities of carnosine, L-histidine, β-alanine, Mannitol and Benzoate were studied with TBA method. The protecting mechanism of carnosine in DNA oxidant damage was analyzed. The results suggested that carnosine has a better protective effect than Mannitol and Benzoate in the right system. Its antioxidant curve is similar to L-histidine, which implied that the antioxidant ability of carnosine is closely bound up with its imidazole cycle.
出处
《河南工业大学学报(自然科学版)》
CAS
北大核心
2006年第1期18-20,共3页
Journal of Henan University of Technology:Natural Science Edition
基金
河南省杰出青年科学基金资助项目(0312001500)
教育部科学技术研究重点项目(204086)