白细胞介素10基因转染对心脏移植排斥反应中细胞黏附分子表达的影响

Effect of Interleukin-10 Gene Transfer on Expression of Cell Adhesion Molecules in Mice Heart Transplantation Rejection

目的探讨白细胞介素10(IL-10)基因转染对小鼠心脏移植排斥反应中细胞黏附分子CD44、选择素E、淋巴细胞功能相关抗原-1(LFA-1)、血管细胞黏附分子-1(VCAM-1)表达的影响。方法采用小鼠颈部心脏移植模型,将96只小鼠用随机数字表法分为3组,对照组:供、受者各16只,均为C57小鼠;移植组:供、受者各16只,分别为BALB/C、C57小鼠;IL-10组:供、受者各16只,分别为BALB/C、C57小鼠,用IL-10重组腺病毒载体先转染供心。3组均行颈部心脏移植。于术后第5 d取移植心脏,用逆转录聚合酶链反应(RT-PCR)法观察CD44、选择素E、LFA-1、VCAM-1及IL-10 mR-NA的表达情况。结果PCR产物电泳及条带密度扫描显示,移植组CD44、选择素E、LFA-1、VCAM-1与对照组比较表达明显增强(P<0.01);IL-10组与移植组比较表达明显降低(P<0.01)。结论IL-10基因转染对心脏移植排斥反应的免疫抑制作用可能与其抑制CD44、选择素E、LFA-1、VCAM-1等细胞黏附分子的表达有关。

Objective To investigate the effect of interleukin-10 （IL-10） gene transfer on expression of CD44, selectin-E, lymphocyte function associated antigen-1 （LFA-1）, vascular cell adhesion molecule-1 （VCAM-1） in mice heart transplantation rejection. Methods Model of mice cervical heterotopic heart transplantation was set up, 96 mice were divided into three groups with random number table, control group： heart transplantation between C57 mice; transplant group： heart from BALB/C mice transplant to C57 mice; IL-10 group： IL-10 was transfected on BALB/C mice isolated heart for 1 hour, then transplanted to C57 mice. The messenger ribonucleic acid （mRNA） level expression of CD44 ,selectin-E ,LFA-1 ,VCAM-1 and IL-10 were measured by reverse transcription-polymerase chain reaction （RT-PCR） at the 5th day after transplantation. Results The mRNA level expression of CD44, selectin-E ,LFA-1 ,VCAM-1 in transplant group were significantly increased than those in control group （P〈0.01）. The mRNA level expression of CD44, selectin-E, LFA-1 ,VCAM-1 in IL-10 group were significantly decreased than those in transplant group （P〈0.01）. Conclusion IL-10 gene transfer is able to decrease the expression of CD44, selectin-E,LFA-1 ,VCAM-1 and suppress the heart transplantation rejection in mice.