藻酸双酯钠对实验性脑缺血大鼠急性期的干预

The Intervention of Polysaccharidesulfate on the Acute Period in the Experimental Cerebral Ischemia Rats

目的探讨不同给药时间藻酸双酯钠对局灶性脑缺血再灌注大鼠脑组织神经细胞凋亡的保护机制。方法经大鼠颈内动脉将一线栓插入右侧大脑中动脉1.5 h后再灌注24 h制成局灶性脑缺血再灌注大鼠模型。在再灌前30 min或再灌注后5 h经腹腔给予相同剂量的藻酸双酯钠。流式细胞术测定受损脑组织神经元细胞内钙离子浓度及凋亡率,同时观察大鼠的神经功能障碍评分。结果藻酸双酯钠治疗组神经功能障碍较非治疗组明显减轻,相应的藻酸双酯钠治疗组细胞内钙离子浓度增高受抑制、细胞凋亡减少。不同给药时间点组间上述指标差异亦有显著性。结论藻酸双酯钠可以减轻脑组织神经元再灌注损伤,抑制神经元凋亡。抑制受损神经元胞内钙离子浓度增高是其保护作用的可能机制。

Aim To study the protective effect of polysaccharidesulfate on cell apoptosis in focal cerebral Ischemia - Repeffusion injury rats. Melhods Right middle cerebral artery was occlude by inserting a thread through internal carotid artery for 1 h and 30 min, and then reperfused for 24 h. PSS were injected via intraperitoneal of the rats at 30 minutes before or 5 hours after reperfusion. Intra-eellular free calcium （ [Ca^2＋ ] i） and rates of apoptosis maid death were measured by flow eytometry. The behavior of rats was investigated at the same time. Results The damage of rat＇s behavior in the PSS-treatment groups was small in comparison with untreated group. The numbers of apoptosis cells were dramatically reduced in the PSS-treatment groups in comparison with untreated group. While the increasing [ Ca^2＋ ]i was reduced by PSS. These significant differenee between 30 min before onset and 5 h after reperfusion groups was also discovered. Conclusions PSS reduced the neuronal damage and inhibited apoptosis induced by ischemia-reperfusion. The anti-apoptosis effects of it might be attributed to its effects by bating the increasing of [ Ca^2＋ ]i.