内皮素1对人血管内皮细胞基因表达谱的影响

Effects of Endothelin-1 on the Gene Expression of Human Vascular Endothelinal Cells

目的探讨内皮素1对人血管内皮细胞基因表达谱的影响,进一步阐明内皮素1导致内皮细胞功能异常的分子机制。方法培养人血管内皮细胞,提取总RNA,采用人内皮细胞生物学功能基因芯片(含96个基因),检测内皮素1作用12 h后人血管内皮细胞主要功能基因表达谱的变化。结果与未用内皮素处理的对照组相比,人血管内皮细胞在内皮素1作用12 h后,共有53个基因出现差异表达,其中抗凝血和抗氧化基因等22个基因表达下调,促凝和炎性因子基因等31个基因表达上调。结论内皮素通过诱导人血管内皮细胞凝血和纤溶系统基因表达失衡、下调抗氧化酶基因表达和上调促炎因子基因表达,损伤人血管内皮细胞,造成其生物学功能异常。

Aim To explore the influence of endothelin-1 （ ET-1 ） on the gene expression of human vascular endothelial cells and the molecular mechanism of vascular endothelial cells injury induced by ET-1. Methods ECV304 cells were cultured and the total RNA was extracted. The GEArry Q Series Human Endothelial Cell Biology Gene Array was used to analyze the differential expression of genes associated with the major functions of endothelial cells 12 hours after treated with ET-1, Resuits Compared with control group,expression of 53 genes was differentiated at the time point of 12-hour in endothelin group, Among them.expression of 22 genes including mainly antithrombotic and antioxidant genes was downregulated and that of 31 genes including mainly prothrombotic and inflammatory factor genes was upregulated, Conclusions ET-1 could injure human vascular endothelial ceils by downregulating antithrombotic and antioxidant genes and upregulating prothrombotic and inflammatory factors genes.