通脉颗粒对兔动脉粥样硬化模型血脂水平及主动脉和冠状动脉病变的影响

The Effects of Tongmai Granules on Blood Lipids,Aorta and Coronary Artery Pathological Morphology in Atherosclerosis Rabbit Model

目的观察通脉颗粒对动脉粥样硬化兔模型血脂水平及主动脉和冠状动脉病变的影响。方法将40只日本大耳白兔随机分为空白对照组、模型对照组、通脉颗粒小剂量组、通脉颗粒大剂量组,每组10只,空白组普通饲料喂养,其余各组予高脂饮食(含5%猪油、0.5%胆固醇)喂养12周复制成动脉粥样硬化模型,其中后两组同时予大小剂量通脉颗粒灌胃,观察各组动物实验前后血脂水平变化及主动脉和冠状动脉病理形态改变(包括主动脉粥样斑块厚度、斑块覆盖面积和冠状动脉狭窄状况)。结果各模型组动物在实验结束时血浆甘油三酯、总胆固醇、低密度脂蛋白均明显上升,但通脉颗粒干预组上升幅度低于模型组,其中大剂量组上升幅度最小,与其他各组比较有显著性差异(P<0.05);空白对照组主动脉、冠状动脉正常,其余各组均有不同程度粥样斑块形成和冠状动脉狭窄,主动脉斑块厚度、斑块覆盖面积占整条主动脉面积的百分比以及狭窄冠状动脉百分比,大小剂量组均比模型组小,而大剂量组最小(P<0.05)。结论通脉颗粒可显著降低动脉粥样硬化兔模型的血脂水平、延缓动脉粥样斑块的发生发展,这可能是该药治疗冠心病心绞痛的重要机理之一。

Aim To observe the effects of Tongmai granules on blood lipids, aorta and coronary artery pathological morphology in atberosclerosis rabbit model. Methods Forty Japanese big ear rabbits were randomly divided into negative control group, model control group, low dosage TongMai granules group and high dosage TongMai granules group, each group consisting ten rabbits. The negative control group was fed on common diet,the other groups were high-lipid fed （5% pork fat, 0.5% cholesterol） to establish atherosclerosis model. The latter two groups were given low dosage and high dosage Tongmai granules respectively. Blood lipid, aorta and coronary artery pathological morphology （including the thickness and the area of the aorta atherosclerosis plaque and the percentage of stenosis of the coronary artery supplying left ventricle ） were observed in all the rabbits before and/or after the treatment. Results Blood TG, TC, LDLC were elevated significantly in the rabbits of the model group, but the elevation in the Tongmai group was lower than those of the model group, especially in the high dosage group. There are significant differences as compared with those of other groups （ P 〈 0.05 ）. Normal aorta and coronary arteries are seen in the negative control group while in the other groups atberosclerosis of various degrees and coronary arteries stenosis came into being. Aorta plaque thickness, plague area/ aorta area and the percentage of stenosis of coronary arteries is lower in the Tongmai gronp than those in the model group , with those of the high dosage group being tbe lowest （P〈0.05）. Conclusions Tongmai granules can significantly reduce blood lipids, prevent and treat atberosclerosis. The mechanisms by which Tongmai granules alleviates angina pectofis may be attributed to its properties of reducing blood lipids, preventing and treating atherosclerosis.