一氧化氮阿司匹林对血小板聚集功能的影响

The Antiplatelet Aggregation Effects of Nitric Oxide-Releasing Aspirin

目的研究一种能缓慢释放一氧化氮的新型阿司匹林(BPI-1096)对血小板聚集功能的影响。方法正常人富血小板血浆与药物在37℃条件下温育10 min后,采用比浊法测定不同诱导剂作用下血小板聚集率。结果不同浓度条件下BPI-1096能够不同程度地降低由二磷酸腺苷、血小板活化因子、肾上腺素及瑞斯托霉素诱导的血小板聚集(P<0.05),对花生四烯酸诱导的血小板聚集虽有降低但是无统计学意义;在相同浓度条件下BPI-1096与阿司匹林抑制血小板聚集率的作用无显著性差异;在不同浓度条件下BPI-1096对血小板聚集率不存在明显的浓度效应关系。结论BPI-1096作为一种新型的一氧化氮阿司匹林,能够显著抑制二磷酸腺苷、血小板活化因子、瑞斯托霉素及肾上腺素等多种诱导条件下体外血小板聚集功能,作用强度与传统阿司匹林疗效相当,其对血小板的抑制作用强度无明显的浓度效应关系。

Aim To study the effects of BPI-1096, a nitric oxide-releasing aspirin, on platelet aggregation. Methods Platelet-rich plasma （PRP） samples was prepared from healthy adult volunteers. The antiaggregant effects of BPI-1096 were tested by a standard nephelometric technique after incubating the drug with platelet at 37℃ for 10 min. Results The platelet aggregation induced by adenosine diphosphate （ ADP）, platelet activating factor （PAF）, adnephrin and restocetin was inhibited by BPI-1096 at different concentration （ P 〈 0.05）. BPI-1096 inhibited platelet aggregation induced by arachidonic acid in every concentration but did ont show any significance （ P 〉 0.05）. BPI-1096 displayed inhibitory effects similar to ASA at the same concentration. BPI-1096 did not strengthen the inhibitory effects on platelet aggregation at high concentration. Conclusions As a newly nitric oxide-releasing aspirin, BPI-1096 can significantly inhibit platelet aggregation induced by adnephrin, restocetin, ADP and PAF in vitro; the antiaggregation effects was similar to Aspirin at the same concentration. BPI-1096 did not show the correlation between concentration and efficiency.