人泌乳素腺瘤中ER、PTTG、bFGF的表达与肿瘤侵袭性的关系

Relationship between invasiveness and expression of ER、PGTT、bFGF in human prolactinomas

目的探讨泌乳素腺瘤中雌激素受体(ER)、垂体瘤转化基因(PTTG)及碱性成纤维因子(bFGF)与肿瘤侵袭生长的关系。方法选择手术切除的泌乳素腺瘤标本46例,分为侵袭组和非侵袭组,采用免疫组化方法分别测定ER、PTTG和bFGF蛋白表达;从组织中提取RNA,经RT-PCR方法测定ER-mRNA和PTTG-mRNA。结果侵袭组ER、PTTG和bFGF蛋白表达的积分吸光度(IA)值分别为5385.1±1348.6、9874.2±2143.7和7938.5±1436.5,ER-mRNA和PTTG-mRNA吸光度比值分别为0.71和0.83,与非侵袭相比较差异显著(P<0.05)。结论ER、PTTG和bFGF与肿瘤的侵袭性密切相关,对肿瘤的发生,发展有重要作用。

Objective To explore the relationship between invasive growth and expression of ER, PTTG ,bFGF in human prolactinomas. Methods Fourty-six prolactinomas specimens were collected and divided into invasive group and non-invasive group. Expressions of ER, PTTG ,bFGF were examined by immunocytochemical method. Total RNA was extracted from specimens and ER-mRNA and PTTG-mRNA were detected by RT-PCR. Results IA of protein expression of ER, bFGF, PTTG in invasive prolactinomas was seperately 5385.1 ± 1348.6,9874.2 ± 2143.7,7938.5 ±1436.5; The optical density ratio of ER-mRNA and PTTG-mRNA was 0.71 and 0.83, difference between invasive group and non-vasive group was significant. Conclusions ER, PTTG, bFGF in human prolactinomas closely correlated with invasiveness of prolactinomas, and may play important function in prolactinomas.