灭活的双歧杆菌治疗菌群失调致小鼠肠源性感染

Application of heat-killed bif idobactera in treatment of enterogenic infection caused by dysbacteriosis in mice

目的:观察灭活的双歧杆菌对菌群失调致小鼠肠源性感染的治疗作用.方法:40只昆明种小鼠随机分为4个处理组:活菌组、死菌组、自然恢复组、正常对照组,每组10只.对血清总蛋白(TP)、血清白蛋白(ALB)、血清球蛋白(GLO)、γ-谷氨酰氨转肽酶(GGT)、血清尿素氮(BUN)、乳酸脱氢酶(LDH)、血肌酐(CRE)以及组织匀浆内的示踪菌进行测定.结果:死菌组与自然恢复组比较,TP、GLO明显升高(71.46±2.01g/Lvs64.61±2.16g/L;35.63±1.57g/Lvs330.73±1.03g/L,P<0.05),GGT、BUN则显著降低(2.47±0.28IU/Lvs7.13±1.19IU/L;8.32±0.71mmol/Lvs12.05±0.64mmol/L,P<0.01).死菌组与活菌组、正常对照组比较,LDH明显降低(2561.23±61.40IU/Lvs2951.70±155.61IU/L,2895.27±104.38IU/L,P<0.05).死菌组与自然恢复组比较,肾、肝、肺组织匀浆内肠杆菌数量均明显下降(1.19±0.37,1.56±0.20,1.62±0.15vs2.17±0.97,2.42±0.14,2.20±0.09,P<0.05或P<0.01).结论:双歧杆菌灭活菌悬液具有与活菌悬液相同的抗菌群失调致小鼠肠源性感染作用.

AIM： To observe the curative effect of heat-killed bifidobactera on dysbacteriosis-induced enterogenic infection in mice. METHODS： A total of 40 mice were randomly divided into living bacterium group, heat-killed bacterium group, spontaneous recovery group, and normal control group （10 for each）. After treatment with the corresponding methods, the serum indexes such as total protein （TP）, albumin （ALB）, globulin （GLO）, γ-Glutamyl transpeptidase （GTT）, blood urea nitrogen （BUN）, lactic dehydrogenase （LDH）, creatinine （CER）, and tagged bacterium in the homogenate of the mice were detected. RESULTS： The levels of TP and GLO in the heat-killed bacterium group were significantly increased （71.46± 2.01 g/L vs 64.61±2.16 g/L; 35.63±1.57 g/L vs 330.73± 1.03 g/L, P 〈0.05）, while the levels of GGT and BUN were significantly decreased （2.47±0.28 IU/L vs 7.13± 1.19 IU/L; 8.32±0.71 mmol/L vs 12.05±0.64 mmol/L,P 〈0.01） as compared with those in the spontaneous recovery group. The level of LDH in the heat-killed bacterium group was significantly decreased as compared with that in the living bacterium group and the normal control group （2 561.23±61.40 vs 2 951.70±155.61, 2 895.27±104.38, P 〈0.05）. The numbers of the enterobactera was markedly deceased in the renal, hepatic and pulmonary tissues of the heat-killed bacterium group in comparison with those in the spontaneous recovery group（1.19±0.37, 1.56±0.20, 1.62±0.15 vs 2.17±0.97, 2.42±0.14, 2.20±0.09, P 〈0.05 or P 〈0.01）. CONCLUSION： The heat-killed bifidobactera are effective in the treatment of enterogenic infection caused by dysbacteriosis in mice, and the effect is the same as that of the living bifidobactera.