摘要
瞄准:在细胞毒素的T淋巴细胞抗原 4 以内估计三个多型性区域( CTLA-4 )基因,在倡导者区域 -318 的 C/T 基础交换( CTLA-4 -318C/T),在 1 个位置上的前的 A/G 替换 49 ( CTLA-4 49A/G ),在 1172 的 T/C 替换(在有长期的肝炎 B 的病人的 CTLA-4 -1172T/C)。方法:当他们介绍了给肝的诊所,有长期的肝炎 B 感染和 150 个健康题目的 51 个病人顺序被招募。长期的肝炎 B (HBV ) 的分类感染了病人作为无征状的带菌状态(26 个病人) 和长期的肝炎 B (25 个病人) 。Genomic DNA 用 Milleros 腌外面方法从凝结反的外部血 Buffy 被孤立。CTLA-4 基因多型性的存在用聚合酶链反应扩大被决定倔强的变化系统(手臂) 。结果:我们观察了在 -318 遗传型频率之间的一个重要协会(T+C- , T+C+ , T-C+) 并且危险性到长期的肝炎 B (P=0.012, OR=0.49, 95%CI:0.206-1.162 ) 。然而,我们没为 +49 遗传型频率观察一个重要协会(T+C+ , T+C-T-C+) 并且 -1172 遗传型频率(C+T+ , T+C-C+T-) 并且疾病的状态。结论:我们的结果建议 CTLA-4 基因多型性可以部分涉及危险性到长期的肝炎 B。
AIM: To assess the three polymorphJsm regions within cytotoxic T-lymphocyte antigen 4 (CTLA-4) gene, a C/T base exchange in the promoter region-318 (CTLA-4 -318C/T), an A/G substitution in the exon 1 position 49 (CTLA-4 49A/G), a T/C substitution in 1172 (CTLA-4 -1172T/C) in patients with chronic hepatitis B.
METHODS: Fifty-one patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to the hepatic clinic. Classification of chronic hepatitis B virus (HBV)-infected patients was as asymptomatic carrier state (26 patients) and chronic hepatitis B (25 patients). Genomic DNA was isolated from anti-coagulated peripheral blood Bully coat using Miller's salting-out method. The presence of the CTLA-4 gene polymorphisms was determined using polymerase chain reaction amplification refractory mutation system (ARMS).
RESULTS: We observed a significant association between -318 genotypes frequency (T+C-, T+C+, T-C+) and susceptibility to chronic hepatitis B (P=0.012, OR=0.49, 95%CI: 0.206-1.162). However, we did not observe a significant association for +49 genotype frequency (T+C+, T+C- T-C+) and -1172 genotype frequency (C+T+, T+C- C+T-) and state of disease.
CONCLUSION: Our results suggest that CTLA-4 gene polymorphisms may partially be involved in the susceptibility to chronic hepatitis B.