Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats

AIM： To evaluate the hepatoprotective effect of garlic on liver injury induced by isoniazid （INH） and rifarnpicin （RIF）. METHODS： Wistar rats weighing 150-200 g were treated orally with 50 mg/kg of INH and RIF daily each for 28 d. For hepatoprotective studies, 0.25 g/kg per day of freshly prepared garlic hornogenate was administered orally half an hour before the INH＋RIF doses. Serum alanine arninotransferase （AIT）, aspartate aminotransferase （AST） and bilirubin were estimated on d 0, 14, 21, and 28 in all the rats. Histological analysis was carried out to assess the injury to the liver. Lipid peroxidation （1PO） as a marker of oxidative stress and non-protein thiols （glutathione） for antioxidant levels were measured in liver hornogenate. RESULTS： The treatment of rats with INH＋RIF （50 mg/kg per day each） induced hepatotoxicity in all the treated animals as judged by elevated serum ALT, AST, and bilirubin levels, presence of focal hepatocytic necrosis （6/8） and portal triaditis （8/8）. Garlic simultaneously administered at a dose of 0.25 g/kg per day prevented the induction of histopathological injuries in INH＋RIF co-treated animals, except in 4 animals, which showed only moderate portal triaditis. The histological changes correlated with oxidative stress in INH＋RIF treated animals. The group which received 0.25 g/kg per day garlic hornogenate along with INH＋RIF showed higher levels of glutathione （P〈0.05） and low levels of 1PO （P〈 0.05） as compared to INH＋RIF treated group. CONCLUSION： Freshly prepared garlic homogenate protects against INH＋RIF-induced liver injury in experimental animal model.

瞄准：为了在肝损伤上评估大蒜的 hepatoprotective 效果，由异菸肼(ZNH ) 和 rifampicin (RIF ) 导致了。方法：称 150-200 g 的 Wistar 老鼠为 28 d 与 INH 和 RIF 每日的各个的 50 mg/kg 口头上地被对待。为 hepatoprotective 研究， 0.25 g/kg 口头上地每刚准备的大蒜匀浆的天被管理在 INH+RIF 剂量前的半个小时。浆液丙氨酸 aminotransferase (中高音) ， aspartate aminotransferase (著名计算机生产厂商) 和胆红素在 d 上被估计 0， 14， 21，和 28 在所有老鼠。组织学的分析被执行估计损害到肝。每氧化(LPO ) ，作为氧化应力和非蛋白质的一个标记，为抗氧化剂的 thiols (谷胱甘肽) 铺平的类脂化合物在肝匀浆被测量。结果：有 INH+RIF 的老鼠的处理(50 mg/kg 每天每) 在由提高的浆液中高音，著名计算机生产厂商，和胆红素判定了的所有对待的动物的导致的 hepatotoxicity 铺平，焦点的 hepatocytic 坏死(6/8 ) 的存在和门 triaditis (8/8 ) 。同时每天在 0.25 g/kg 的剂量管理的大蒜在 INH+RIF 阻止了组织病理学说的损害的正式就职共同对待的动物除了在 4 个动物，它显示出仅仅中等的门 triaditis。组织学的变化在 INH+RIF 与氧化应力相关对待的动物。作为与 INH+RIF 相比与谷胱甘肽(P<0.05 ) 和 LPO (P<0.05 ) 的底层高级的显示出的 INH+RIF 一起每天大蒜匀浆收到了 0.25 g/kg 的组对待组。结论：刚准备的大蒜匀浆在实验动物模型免于导致 INH+RIF 的肝损伤。