缬沙坦对豚鼠心肌缺血时去甲肾上腺素释放的影响

Effect of valsartan on cardiac norepinephrine release under ischemic conditions

目的探讨在缺血条件下不同浓度缬沙坦对豚鼠离体心脏去甲肾上腺素(NE)释放的影响及机制。方法建立豚鼠离体心脏缺血模型,以高效液相色谱仪测定缬沙坦加入前后NE和二羟基苯乙二醇(DHPG)含量的变化,并探讨它们之间的关系。结果①在缺血条件下NE释放量较正常氧供时明显增加;②在缺血10min后,中、高浓度缬沙坦灌注组NE释放量较缺血前明显增加,并呈浓度依赖性,随缬沙坦灌注浓度增加NE释放明显增加;③在缺血10min后,中、高浓度缬沙坦灌注组DHPG释放量较缺血前明显减少,并呈浓度依赖性,随缬沙坦灌注浓度增加DHPG释放明显减少。结论缬沙坦促进豚鼠离体心脏短时间(10min)缺血条件下时的NE释放并呈剂量依赖性。

Objective To investigate the effects and mechanism of valsartan at different concentrations on cardiac norepinephrine release in isolated perfused guinea pig heart under ischemic conditions. Methods A guinea isehemie heart model was established. High-performance liquid chromatographywas used to determine the outflow of cardiac NE and dihydroxyphenylglyi col （DHPG） from isolated perfused guinea pig hearts. ：Results ONE release was significantly increased under ischemic condition as compared with non-ischemie condition. ① After 10 min ischemia, NE release was significantly increased in valsartan middle and high concentration groups as corhpared with control group under non-ischemic condition in a concentration dependent manner, ② After 10 min isehemia, DHPG release was markedly decreased in valsartan middle and high concentration groups as compared with control group under non-ischemie condition in a concentration dependent manner. Conclusion Valsartan can promote norepinephrine release in a concentration depeadent manner during short-term ischemia （10 min）.