低密度脂蛋白受体基因多态性及其与慢性丙型肝炎的相关性

Association between polymorphism of human low-density lipoprotein receptor gene and chronic viral hepatitis C in Chinese patients

目的探讨我国汉族正常人群及慢性丙型肝炎(chronicviralhepatitisC,CHC)患者低密度脂蛋白受体(LDL-R)基因外显子12中HincⅡ多态性的分布及其与慢性丙型肝炎之间的关系。方法采用聚合酶链反应结合限制性片段长度多态性(PCR-RFLP)的方法,对84例慢性丙型肝炎病毒(HCV)感染者和72名健康献血员进行LDL-R基因HincⅡ多态性检测。结果在HCV感染组和健康对照组、慢性丙型肝炎组和HCV相关肝硬化组、丙氨酸转氨酶(ALT)正常组和ALT升高组,以及HCVRNA阴性组和HCVRNA阳性组之间,LDL-R基因外显子12HincⅡ多态性分布差异无统计学意义。结论人群LDL-R基因外显子12中存在着HincⅡ多态性位点,但该多态性位点可能与慢性HCV感染无相关性。

Objective To detect Hinc Ⅱ polymorphism in low-density lipoprotein receptor（LDL-R） gene,and to study its relation to chronic viral hepatitis C （CHC） in the Han nationality of China.Methods Polymerase chain reaction restriction fragment length polymorphisms（PCR-RFLP） method was used to detect Hinc Ⅱ polymorphism of the LDL-R gene in 84 patients infected with HCV. and 72 healthy blood donors.Results There was no statistically significant differences in Hine Ⅱ polymorphism of the LDL-R gene exon 12 between patients infected with HCV and healthy controls,between patients with chronic viral hepatitis C and those with HCV associated cirrhosis,between patients with normalized serum ALT and those with abnormal ALT level, and between HCV RNA negative and positive patients.Conclusion There is Hinc Ⅱ polymorphism in the LDL-R gene exon 12 in the Han natiancity poputation,but it may not be involved in the chronic viral hepatitis C.