国产抗HIV－1试剂盒对献血员血清检测

Using Domestic Anti-HIV-1 Kit to Test Multi-specimens of Blood Donors

国产抗ＨＩＶ－１试剂盒对献血员血清检测陈长荣，戴永强（厦门市中心血站）（厦门新创科技有限公司）艾滋病＋（ＡＩＤＳ）是一种正在全世界广泛传播的疾病，其病毒（ＨＩＶ）是一种单链ＲＮＡ逆转录病毒．ＨＩＶ感染后２个月左右在血清中可检测出抗ＨＩＶ阳性，从感染到...

Three to five mixed serum specimens were tested by the dometic Anti-HIV-1 EIA kit. The total volume was not changed, but just changed the ratio of the volume of serum specimen to the sample dilution. The results show that there is no difference between one positive specimen and mixed with 2 to 4 negative sera (p>0. 05). The OD of negative control increased with increasing the number of mixed serum specimen, but the OD of all negative mixed serum were below to cutoff. So it is no effect to the kit specificity by the mixed serum (3 or 5 samples).And all the different concentration of Pasteur's standard cut off sera could be tested out. Total 5079 blood donor samples were screened by this method. The number of false positive is 5(0. 098% ). And 5 sera of AIDS patient were tested, the results are all positive. So the false negative ratio is zero.