肾实质性高血压INSR基因第8外显子多态性分析

INSULIN RECEPTOR GENE POLYMORPHISM ANALYSIS IN RENAL HYPERTENSIVE PATIENTS

目的探讨肾实质性高血压胰岛素受体(INSR)基因第8外显子多态性,试图从遗传角度寻求病因。方法采用多聚酶链反应(PCR)和限制性内切酶片段长度多态性分析(RFLP)的方法对38例肾实质性高血压肾功能正常者(RH-NRF)、45例肾实质性高血压肾衰竭者(RH-CRF)及38例正常血压者(NC)全血INSR基因第8外显子NsiⅠ多态性进行分析检测。结果未发现中国人肾实质性高血压INSR基因第8外显子NsiⅠ多态性改变,两病人组N2等位基因频率与正常对照组比较无显著性差异(2χ=0.06、0.08,P>0.05)。结论INSR基因第8外显子NsiⅠ多态性与中国人肾实质性高血压无关,N2等位基因并非其易感基因。

Objective To investigate the relationship between polymorphism of insulin receptor （INSR） gene Exon 8 and the etiology of renal hypertension. Methods Polymerase chain reaction （PCR） combined with restriction fragment length polymorphism （RFLP） was used to detect and analyze the polymorphism of INSR gene Exon 8 in 38 renal hypertensive patients with normal renal function （RH-NRF）, 45 patients complicated by chronic renal failure （RH-CRF） and 38 normal controls （NC）. Results No polymorphlsm of Nsi I in INSR gent Exon 8 was found to be correlated with renal hypertension in Chinese. N2 allele frequency of INSR gene had no difference between renal hypertension with or without chronic renal failure （X^2= 0.06, 0.08;P〉 0.05）. Conclusion Nsi I polymorphism of INSR gene Exon 8 has no relation with renal hypertension in Chinese. N2 allele of INSR gene is not the correlated gene of renal hypertension.