自制氟碳声学造影剂对正常兔腹主动脉造影的实验研究(英文)

Experimental study on enhanced ultrasonography of normal abdominal aorta using self-made perfluorocarbon ultrasound contrast agent in rabbits

目的观察自制氟碳声学造影剂对正常兔腹主动脉造影效果及不同造影剂注入方式造影的时间-强度曲线变化。方法经兔耳缘静脉注入0.5mL/kg自制造影剂,常规成像观察实时造影过程,触发成像模式定量分析团注和推注法(0.1mL/s)造影的时间-强度曲线变化。结果造影后腹主动脉灰阶及多普勒信号显著增强。团注法同推注方式比较,峰值强度显著增高,但曲线下降斜率快,平均通过时间短(P<0.001);而推注方式可显著延长造影剂平均通过时间(P<0.001)。结论自制氟碳声学造影剂显著增强血流显像,根据不同检查目的选择合适的造影剂注入方式,对提高造影效果有重要价值。

[Objective] To observe the effect of enhanced uhrasonography on normal rabbit abdominal aorta using self-made perfluorocarbon ultrasound contrast agent and the time-intensity curve changes by different injection methods. [Methods] Self-made contrast agent was injected via rabbit ear vein at a dose of 0.5 ml/kg, real-time abdominal aorta enhancement was observed by conventional imaging, and changes of time-intensity curves were quantitatively analyzed using triggered imaging by bolus injection and infusion （0.1 mL/s）. [Results] Gray-scale and Doppler signal of abdominal aorta were significandy enhanced by contrast agent. Peak intensity and descending slope were much increased by bolus compared with by infusion, but mean transit time was reduced （P 〈0.001）; while mean transit time was longer using infusion （P 〈0.001）. [Conclusion] Self-made perfluorocarbon ultrasound contrast agent can signit]eantly enhance blood flow display,it is importance to choice ideal injection method according to checking aim to improve the effect of enhanced uhrasonography.