环氧合酶-2在卵巢浆液性肿瘤中的表达与其致瘤性的研究

Expression of COX-2 in ovarian serous tumors and the study of its tumorgenesis mechanisms

目的研究环氧合酶-2(cyclooxygenase-2,COX-2)蛋白和Fas、FasL以及血管内皮生长因子受体2(vescularendotheliumgrowthfactorreceptor-2,又称KDR)在正常卵巢和卵巢浆液性肿瘤中的表达及其临床意义。方法应用免疫组化法检测COX-2蛋白和Fas、FasL、KDR在56例卵巢浆液性肿瘤(其中良性9例,交界性9例,癌38例)及8例正常卵巢组织的新鲜标本中的表达。结果COX-2在卵巢浆液性癌(ovarianserouscarcinoma,OSC)及交界性瘤中表达阳性率分别为84%和89%,明显高于正常组织及良性瘤组织,差异有显著性(P<0.05)。在OSC中,Fas表达明显降低,FasL表达上升,而KDR表达明显升高,与COX-2表达有协同性。结论COX-2蛋白与卵巢浆液性肿瘤发生发展密切相关,COX-2蛋白可能通过导致肿瘤细胞凋亡异常以及促血管生成而促进肿瘤发生、发展。

[Objective] To study the expression of COX-2, Fas, FasL and KDR in normal ovarian tissues, ovarian serous tumors and its clinical significance. [Methods] 56 flash samples with benign, borderline and malignant ovarian serous tumors and 8 samples of normal ovarian tissues were detected for their expression of COX-2 and Fas, FasL KDR by SP immunohistochemical technique. [Results] The positive expression rate of COX-2 in the ovarian serous carcinoma（OSC）and the borderline tumor were 84% and 89%, respectively. And they were significantly higher than that in benign （0%） and normal ovarian tissues （0%） （P 〈0.05）. In OSC, the expression of Fas was down-regulation, the expression of FasL was up-regulation, the expression of KDR was up-regulation. [ Conclusion] COX-2 is up-regulated in the ovarian borderline tumor and OSC. COX-2 may promote tumorogenesis by inhibiting apoptosis by Fas/FasL system and promoting angiogenosis.