血清自体荧光法诊断恶性肿瘤的研究

Cancer diagnosis by serum autofluorescence

目的研究癌患者血清自体荧光光谱的特征,建立简便快速的癌筛查方法,为实现肺癌,肠癌的早期发现提供辅助手段。方法用常规的荧光分光光度计,在405nm激发下,测量癌患者和正常人血清的自体荧光发射光谱,分析研究二者的荧光光谱差异。结果与正常人的血清自体荧光相比,肺癌,肠癌和乳腺癌患者在518nm的荧光峰明显增强(P<0.004);肺癌和肠癌患者血清在622nm荧光峰的相对强度T与背景荧光I612(612nm处荧光强度)有关。当I612在6≤I612<9(×105a.u.)范围,癌患者的T指标显著高于对照组的(P<0.02);在其他区域,癌患者与对照组差异无显著性(P>0.05)。根据上述特性建立了癌诊断判据,对肺癌和肠癌的阳性率分别为87%和83%,特异性85%。结论本方法对肺癌和肠癌的筛查具有潜在价值,值得进一步研究。

[Objective] To investigate differences in serum autofluorescence between healthy and cancer people and establish simple cancer screening method. [Methods] Measure serum autofluorescence spectra of healthy and cancer people with excitation wavelength of 405 nm by use of conventional fluorescence spectrophotometer, and analyze fluorescence spectrum differences between cancer and healthy people. [Results] Fluorescence peak intensities at 518 nm of lung cancer, colon cancer and breast cancer people were significantly higher than those of healthy people （P 〈0.004）; relative fluorescence intensity T at 622 nm of lung cancer and colon cancer patients were related to background I612 （fluorescence intensity at 612 nm）： in the range of 6≤I612〈9×10^4a.u.）, the index T of cancer patient was significandy greater than that of normal people （P 〈0.02）; in other range there were no significant differences between them （P 〉0.05）. The cancer diagnosis criterion was established with sensitivities of 87% and 83% for lung cancer and colon cancer respectively, and specificity of 85%. [Conclusions] Detecting serum autofluorescenc is of potentially value for screening lung cancer and colon cancer and worth studying further.