亚硒酸钠和硫杂脯氨酸组合对人胃癌细胞的诱导分化作用

Induced Differentiation of Human Gastric Adenocarcinoma Cells by Sodium Selenite and Thioproline

以亚硒酸钠和硫杂脯氨酸为诱导剂组合，处理人胃腺癌ＭＧｃ８０－３细胞．通过细胞生长曲线和分裂指数的测定、光镜细胞化学和酶标法检测碱性磷酸酶活性，以及免疫胶体金技术标记ｃ－ｅｒｂＢ－２癌基因蛋白等方法，证实诱导剂组合能有效地抑制ＭＧｃ８０－３细胞恶性生长，改变碱性磷酸酶活性和分布，引起ｃ－ｅｒｂＢ－２癌基因蛋白表达的变化，具有一定的诱导分化作用．

A human gastric adenocarcinoma cell line MGc80-3 was induced by sodium selenite (3×10-6mol/L)and thioproline (1. 5×10-4mol/L). Through examing the growgh rate and mitotic index, assaying the activity and distribution of alkaline phosphatase by enzymic labelling and cytochemistry methods,and detecting onco-protein of cerbB-2 by Colloidal Gold immunolabelling technique, it was showed that sodium selenite and thioproline could inhibit the growth of MGc80-3 cells effectively, decrease the activity and change distribution of alkaline phosphatase,and inhibit the expression of onco-protein of cerbB-2. Sodium selenite and thioproline can play a role of induced differentiation on human gastirc adenocarcinoma cells.