伤寒杆菌L型与其细菌型抗原性比较

COMPARATIVE STUDIES ON THE ANTIGENICITIES OF SALMONELLA TYPHOSA AND ITS L-FORMS

本文用因子血清检查伤寒杆菌H901株细菌型及L型的抗原结构,结果细菌型除Vi抗原缺失外,O和H抗原均完整;L型O_9抗原检测不出,但与AFO多价血清呈弱阳性,可能另有少量其他O抗原,如O_(12)抗原存在,H抗原仍保留不变。以伤寒杆菌L型和细菌型分别免疫家兔,将所获得的免疫血清与其相应的H与O抗原进行试管凝集1试验,结果L型免疫血清的O抗体及H抗体的凝集效价较细菌型的低(P<0.01,P<0.001),表明L型的抗原性降低。用L型抗原与细菌型免疫血清以及用细菌型抗原与L型免疫血清进行交叉凝集试验,两者之间有一定交叉凝集。而交叉凝集吸收,则见细菌型抗原能吸收掉L型免疫血清中全部的抗体,L型抗原却只能吸收细菌型免疫血清中部分抗体。

In this article, O antigens of Salmonella typhi H901 L-forms were tested with monovalcnt and AFO polyvalent antisera. No O9 anti-gen specific for S. typhi was found with monovalent antiserum on S. typhi H 901 L-forms, and L-forms could react but weakly with AFO polyvalent antiserum. O12 might still be present. Animals were immunized with vegetative and L-forms of S. typhi and the immune sera obtained were examined with the agglutination test. The liters of anti-L-form serum were much lower than these of anti-vegetative forms when tested with O and H antigen (P<0.01,P<0.001). Cross absorption tests were made with two antigen-antibody pairs. Vegetative forms could absorb all antibodies from the anti-L-form serum, but not in the reverse way. It indicates that there are still some O antigens left.