活性氧自由基对细胞色素C和肌红蛋白的作用

The Tudie on the Injure of Cytochrome C and Myoglobin by Active Oxygen Radical

研究了过硫酸铵－Ｎ，Ｎ，Ｎ＇，Ｎ＇四甲基乙二胺（ＡＰ－ＴＥＭＥＤ）体系产生的活性氧自由基（超氧阴离子自由基（Ｏ２－）、羟基自由基（·ＯＨ））对细胞色素Ｃ和肌红蛋白的作用．结果表明，Ｏ２－可以将细胞色素Ｃ中的三价铁离子还原为二价铁离子，延长作用时间可使铁卟啉基团脱落；亦可使肌红蛋白中的铁卟啉脱落．与此同时，紫外可见吸收光谱发生了变化．圆二色谱的变化说明蛋白分子中的α－螺旋遭到破坏．植物Ｌ－ＳＯＤ对于该损伤有保护作用．

The active oxygen radicals generated by AP-TEMED system can affect the structure and quality of cytochrome C (Cyt C) and myoglobin .(Myo). The results have proved that active oxygen radicals can reduce the Fe3+, which exists in the oxidative Cyt C, to Fe2+. And after sometime the ferroprotoporphyrin will be deprived from the Cyt C. The active oxygen radicals can also make the deprivation of ferroprotoporphyrin from Myo. At the same time, the absorption spectrum is changed. The changes of CD spectrum demonstrate that α-helix structure has been damaged. The L-SOD extracted from plants can prevent the injury.