异鼠李素对AngⅡ诱导新生大鼠心脏成纤维细胞增殖和胶原合成的影响

Influence of isorhamnetin on cultured neonatal rat cardic fibroblast proliferation and collagen synthesis induced by angiotensinⅡ

目的探讨异鼠李素(Isorhamnetin)对血管紧张素Ⅱ(AngⅡ)诱导新生大鼠心脏成纤维细胞(CFb)增殖和胶原合成的影响。方法建立AngⅡ诱导新生大鼠CFb纤维化模型,采用MTT比色法检测CFb增殖;采用3H-脯氨酸掺入法检测CFb胶原合成,分别观察不同浓度异鼠李素对CFb增殖和胶原合成的影响。结果在一定范围内,异鼠李素以浓度依赖性方式抑制AngⅡ诱导CFb的增殖和胶原合成(P<0.01)。结论异鼠李素具有抑制CFb增殖和胶原合成的作用,对心肌纤维化具有一定的防治效应。

Objective To study the influence of Isorhamnetin on cultured neonatal rat cardic fibroblast （CFb） proliferation and collagen synthesis induced by angiotensin Ⅱ. Methods Neonatal rats were treated with angiotensin Ⅱ （AngⅡ） to form cardic fibrosis model. The effects of isorhamnetin on CFb proliferation was measured by thiazolyl blue （MTY） colorimetric assay, synthesis of collagen was measured by 3H-proline incorporation assay. Results Isorhamnetin （10^-4-10^-6mol/L） inhibited angiotensin Ⅱ induced CFb proliferation and collagen synthesis in a dose-dependent manner （P〈0.01）. Conclusion Isorhamnetin inhibited angiotensin Ⅱ induced cardic fibrosis by inhibiting CFb proliferation and collagen synthesis. It suggests that isorhamnetin may play a role in the regression of heart remodeling.